The Localization and Secretion of a Proteolytic Enzyme Complex by the Dermatophytic Fungus Free

Abstract

SUMMARY: The dermatophyte, synthesized a complex of proteolytic enzymes localized in discrete organelles in the mycelium. On induction by protein in the growth medium, the intracellular level rose sevenfold and the enzymes appeared in the culture medium. The process is thought to be true secretion since an autolytic marker enzyme was present in insignificant amounts during the exponential growth phase when the proteases appeared. Amino acids in the medium repressed the synthesis of extracellular proteases.

The protease complex had pH optima at 6·6,8·0 and 9·5 to 10·0 and showed endo-peptidase rather than exopeptidase activity. The possible relevance of such an enzyme system to the pathogenicity and keratinophilia of is discussed.

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1971-11-01
2024-03-28
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References

  1. Anson M. L. 1938; The estimation of pepsin, trypsin, papain and cathepsin with haemoglobin. Journal of General Physiology 22:79–89
    [Google Scholar]
  2. Baxter M., Mann P. R. 1969; Electron microscope studies of the invasion of human hair in vitro by three keratinophilic fungi. Sabouraudia 7:33–37
    [Google Scholar]
  3. Burgum A. A., Prescott J. M. 1965; Isolation and characterization of a fungal endopeptidase. Archives of Biochemistry and Biophysics 3:391–398
    [Google Scholar]
  4. Chattaway F. W., Ellis D. A., Barlow A. J. E. 1963; Peptidases of dermatophytes. Journal of Investigative Dermatology 41:31–37
    [Google Scholar]
  5. Chesters C. G. C., Mathison G. E. 1963; The decomposition of wool keratin BY Keratinomyces ajelloi. Sabouraudia 2:225–237
    [Google Scholar]
  6. Cruikshank C. N. D., Trotter M. D. 1956; Separation of epidermis and dermis by filtrates of Trichophyton mentagrophytes. Nature; London: 1771085
    [Google Scholar]
  7. Day W. C., Toncic P., Stratman S. L., Leeman U., Harmon S. R. 1968; Isolation and properties of an extracellular protease of Trichophyton granulosum. Biochimica et biophysica acta 167:697–606
    [Google Scholar]
  8. English M. P. 1963; The saprophytic growth of keratinophilic fungi on keratin. Sabouraudia 2:115–130
    [Google Scholar]
  9. English M. P. 1968; The developmental morphology of the perforating organs and eroding mycelium of dermatophytes. Sabouraudia 6:218–227
    [Google Scholar]
  10. Grassman W., Heyde W. 1929; Alkalimetrische Mikrobestimmung der Aminosauren und Peptide. Hoppe-Seyler’s Zeitschrift für physiologische Chemie 183:32
    [Google Scholar]
  11. Hofman T., Shaw R. 1964; Proteolytic enzymes of Penicillium janthinellum. I. Purification and properties of a trypsinogen-activating enzyme (peptidase A). Biochimica et biophysica acta 92:543–557
    [Google Scholar]
  12. Iten W., Matile P. 1970; Role of chitinase and other lysosomal enzymes of Coprinus lagopus in the autolysis of fruiting bodies. Journal of General Microbiology 61:301–309
    [Google Scholar]
  13. Keddie F. M. 1967; Further investigations of the electron microscopic examination of human hair naturally infected by Microsporum gypseum. Sabouraudia 6:1–4
    [Google Scholar]
  14. Kunitz M. 1947; Crystalline soybean trypsin inhibitor. Journal of General Physiology 30:291–310
    [Google Scholar]
  15. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  16. Mathison G. E. 1964; The microbiological decomposition of keratin. Annates de la Société beige de médecine tropicale 44:767–792
    [Google Scholar]
  17. Matheson A. T., Tattrie B. L. 1964; A modified Yemm and Cocking reagent for peptidase assay. Canadian Journal of Biochemistry 42:95–99
    [Google Scholar]
  18. Matile P. 1965; Intracellular localization of proteolytic enzymes of Neurospora crassa. I. Function and subcellular distribution of proteolytic enzymes. Zeitschrift für Zellforschung und mikroskopische Anatomic 65:884–893
    [Google Scholar]
  19. Matile P. 1967; Porosity of the cell wall as related to the secretion of proteolytic enzymes in Neurospora crassa. Neurospora Newsletter 12:6
    [Google Scholar]
  20. Matile P., Wiemken P. 1967; The vacuole as the lysosome of the yeast cell. Archiv für Mikrobiologie 56:148–155
    [Google Scholar]
  21. Mercer E. H., Verma B. S. 1963; Hair digested by Trichophyton mentagrophytes. An electron microscope examination. Archives of Dermatology 87:357–360
    [Google Scholar]
  22. O’Sullivan J. 1970 The proteolytic system of Microsporumcanis Ph.D. Thesis University of London:
    [Google Scholar]
  23. O’Sullivan J., Mathison G. E. 1970; Interference by monosaccharides with the estimation of tyrosine and proteins using the Folin-Ciocalteu phenol reagent. Analytical Biochemistry 35:540–542
    [Google Scholar]
  24. Pollock M. R. 1962; Exoenzymes. In The Bacteria IV Gunsalus I. C., Stanier R. Y. Edited by New York: Academic Press;
    [Google Scholar]
  25. Roberts F. F., Doetsch R. N. 1967; Purification of a highly active protease from a Microsporum species. Antonie van Leeuwenhoek 33:145
    [Google Scholar]
  26. Schwert G. W., Neurath H., Kaufman S., Snoke J. E. 1948; The specific esterase activity of trypsin. Journal of Biological Chemistry 172:221
    [Google Scholar]
  27. Smith E. L. 1955; Aminopeptidase (Tripeptidase). In Methods in Enzymology 2 pp. 83–87 Colowick S. P., Kaplan N. O. Edited by New York: Academic Press;
    [Google Scholar]
  28. Weary P. E., Canby C. M., Crawley E. P. 1965; Keratinolytic activity of Microsporum canis and Microsporum gypseum. Journal of Investigative Dermatology 44:300–312
    [Google Scholar]
  29. Yu R. J., Harmon S. R., Blank F. 1969; Hair digestion by a keratinase of Trichophyton mentagrophytes. Journal of Investigative Dermatology 53:166–173
    [Google Scholar]
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