Summary: The dermatophyte, synthesized a complex of proteolytic enzymes localized in discrete organelles in the mycelium. On induction by protein in the growth medium, the intracellular level rose sevenfold and the enzymes appeared in the culture medium. The process is thought to be true secretion since an autolytic marker enzyme was present in insignificant amounts during the exponential growth phase when the proteases appeared. Amino acids in the medium repressed the synthesis of extracellular proteases.

The protease complex had pH optima at 6.6, 8.0 and 9.5 to 10.0 and showed endo-peptidase rather than exopeptidase activity. The possible relevance of such an enzyme system to the pathogenicity and keratinophilia of is discussed.


Article metrics loading...

Loading full text...

Full text loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error