1887

Abstract

SUMMARY

Post-mitochondrial supernatants isolated from contained high specific activities of antimycin A-insensitive NADH- and NADPH- cytochrome oxidoreductases, acid and alkaline p-nitrophenylphosphatases, DNase and ATPase; many of the enzymes associated with mammalian microsomes were not present at detectable levels. The only haem detected was protohaem; cytochromes , and were present in microsomal fractions, and ethyl morphine and aniline HC1 produced distinct changes in difference spectra. Post-mitochondrial supernatants were subfractionated by density gradient centrifugation. The distributions of NADH- and NADPH-cytochrome oxidoreductases were different, and several distinct particles containing the latter enzyme system were distinguished. The distributions of acid -nitrophenylphosphatase, acid DNase and ATPase were all different and indicative of several functionally distinct membrane systems. Alkaline -nitrophenylphosphatase was non-sedimentable.

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/content/journal/micro/10.1099/00221287-68-3-283
1971-11-01
2024-12-13
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References

  1. Anderson N. G., Waters D. A., Fisher W. D., Cline G. B., Nunley C. E., Elrod L. H., Rankin C. T. 1967; Analytical techniques for cell fractionations. V. Characteristics of the BXIV and BXV zonal centrifuge rotors. Analytical Biochemistry 21:235–252
    [Google Scholar]
  2. Cameron I. L., Cline G. B., Padilla G. M., Miller O. L.Jun van Dreal P. A. 1966; Polysomes from Tetrahymena following pyrimidine deprivation and replacement. National Cancer Monograph 21:361–369
    [Google Scholar]
  3. Cartledge T. G., Lloyd D. 1972; Distributions of marker enzymes in aerobically-grown, glucose derepressed Saccharomyces carlsbergensis after zonal centrifugation: effects of EDTA and magnesium ions. Biochemical Journal in the Press
    [Google Scholar]
  4. de Duve C., Berthet J., Beaufay H. 1959; Gradient centrifugation of cell particles. Theory and applications. Progress in Biophysical Chemistry 9:326–369
    [Google Scholar]
  5. Estabrook R. W., Cohen B. 1969; Organization of the microsomal electron transport system. In Microsomes and Drug Oxidations p. 95 Gillette J. R., Conney A. H., Cosmides G. J., Estabrook R. W., Fouts J. R., Mannering G. J. Edited by New York and London: Academic Press;
    [Google Scholar]
  6. Falk J. E. 1964 Porphyrins and Metalloporphyrins New York: Elsevier Publishing Co;
    [Google Scholar]
  7. Halsall H. B., Schumaker V. N. 1969; Sedimentation coefficients in the zonal centrifuge. Analytical Biochemistry 30:368–376
    [Google Scholar]
  8. Hartman H., Dowben R. M. 1970a; Polyribosomes from Tetrahymena. Journal of Cell Biology 44:676–678
    [Google Scholar]
  9. Hartman H., Dowben R. M. 1970b; Polyribosomes profiles from heat synchronized Tetrahymena. Biochemical and Biophysical Research Communications 40:964
    [Google Scholar]
  10. Imai Y., Sato R. 1966; Substrate interaction with hydroxylase system in liver microsomes. Biochemical and Biophysical Research Communications 22:620–626
    [Google Scholar]
  11. Ishidate K., Kawaguchi K., Tagawa K., Hagihara B. 1969; Haemoproteins in anaerobically-grown yeast cells. Journal of Biochemistry, Tokyo 65:375–383
    [Google Scholar]
  12. Klein H. P., Volkmann C. M., Chao F. C. 1967; Fatty acid synthetase of Saccharomyces cerevisiae. Journal of Bacteriology 93:1966–1971
    [Google Scholar]
  13. Leloir L. F., Cardini C. E. 1957; Characterization of phosphorus compounds by acid lability. Methods in Enzymology 3:840
    [Google Scholar]
  14. Lloyd D., Brightwell R., Venables S. E., Roach G. I., Turner G. 1971; Subcellular fractionation of Tetrahymena pyriformis st by zonal centrifugation: changes in activities and distribution of enzymes during the growth cycle and on starvation. Journal of General Microbiology 65:209–223
    [Google Scholar]
  15. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  16. Marcus L., Ris H., Halvorson H. O., Bretthauer R. K., Bock R. M. 1967; Occurrence, isolation and characterization of polyribosomes in yeast. Journal of Cell Biology 34:505–512
    [Google Scholar]
  17. Omura T., Sato R. 1964; The carbon monoxide-binding pigment of liver microsomes. I. Evidence for its haemoprotein nature. Journal of Biological Chemistry 239:2370–2378
    [Google Scholar]
  18. Poole R. K., Nicholl W. G., Turner G., Roach G. I., Lloyd D. 1971; Sedimentation characteristics of mitochondria, peroxisomes and lysosomes from the ciliate protozoon, Tetrahymena pyriformis, strain STafter chloramphenicol-inhibited growth. Journal of General Microbiology 67:161–173
    [Google Scholar]
  19. Reid E. 1967; Membrane systems. In Enzyme Cytology p. 321 Roodyn D. B. Edited by London and New York: Academic Press;
    [Google Scholar]
  20. Sato R., Nishibayashi H., Ito A. 1969; Characterization of two haemoproteins of liver microsomes. In Microsomes and Drug Oxidations p. 111 Gillette J. R., Conney A. H., Cosmides G. J., Estabrook R. W., Fouts J. R., Mannering G. J. Edited by New York and London: Academic Press;
    [Google Scholar]
  21. Schatz G., Klima J. 1964; Triphosphopyridine nucleotide cytochrome c reductase of Saccharomyces cerevisiae: a microsomal enzyme. Biochimica et biophysica acta 81:448–461
    [Google Scholar]
  22. Turner G., Lloyd D., Chance B. 1971; Electron transport in phosphorylating mitochondria from Tetrahymena pyriformis st. Journal of General Microbiology 63:359–374
    [Google Scholar]
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