1887

Abstract

Summary: Post-mitochondrial supernatants isolated from contained high specific activities of antimycin A-insensitive NADH- and NADPH-cytochrome oxidoreductases, acid and alkaline -nitrophenylphosphatases, DNase and ATPase; many of the enzymes associated with mammalian microsomes were not present at detectable levels. The only haem detected was protohaem; cytochromes , and were present in microsomal fractions, and ethyl morphine and aniline HCl produced distinct changes in difference spectra. Post-mitochondrial supernatants were subfractionated by density gradient centrifugation. The distributions of NADH- and NADPH-cytochrome oxidoreductases were different, and several distinct particles containing the latter enzyme system were distinguished. The distributions of acid -nitrophenylphosphatase, acid DNase and ATPase were all different and indicative of several functionally distinct membrane systems. Alkaline -nitrophenylphosphatase was non-sedimentable.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-68-3-283
1971-11-01
2019-10-16
Loading full text...

Full text loading...

http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-68-3-283
Loading
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error