1887

Abstract

SUMMARY

The rates of hydrolysis of carbenicillin and of other penicillins and cephalosporins by nine different -lactamase preparations obtained from Gram-negative bacteria were compared. Enzymes produced by Klebsiella strains, most active against penicillins, as well as -lactamases synthesized by and strains hydrolysed carbenicillin, although at relatively lower rates than ampicillin or cephaloridine. In contrast, carbenicillin was extremely resistant to -lactamases with a predominant cephalosporinase activity as produced by and Enterobacter strains. The cephalosporin -lactamases activity of these enzymes was inhibited by carbenicillin. A considerably increased enzymic activity observed in one strain of when grown in the presence of carbenicillin or other -lactam antibiotics was unable to destroy carbenicillin to any measurable extent. A possible permeability barrier to carbenicillin has been demonstrated in some strains.

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/content/journal/micro/10.1099/00221287-68-3-263
1971-11-01
2022-01-22
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