1887

Abstract

Summary: Paramecia belonging to certain strains of (syngens 1, 2 and 8) were transferred from dual culture with bacteria to axenic media, where the growth of some stocks continued, with weekly subculturing. The only axenically grown stock found to be capable of supporting growth of mu particles indefinitely was stock 138 (syngen 8).

Lambda and mu particles from axenically grown were cultivated in a highly complex medium at 27° under aerobic conditions. The particles retained their characteristic killing action on certain stocks but could not infect sensitive paramecia. The particles divided at approximately one fission per day and achieved a maximum density of only 16–20 × 10/ml. The implications of these studies for the interaction of the nuclear genes and the killer particles are discussed.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-68-3-253
1971-11-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/micro/68/3/mic-68-3-253.html?itemId=/content/journal/micro/10.1099/00221287-68-3-253&mimeType=html&fmt=ahah

References

  1. Baker R. 1970; Studies on the RNA of the mate-killer particles of Paramecium. Heredity 25:657–662
    [Google Scholar]
  2. Balsley M. 1966; Dependence of the kappa particles of stock 7 of Paramecium aurelia on a single gene. Genetics 54:320–325
    [Google Scholar]
  3. Beale G. H., Jurand A. 1960; Structure of the mate-killer (mu) particles in Paramecium aurelia, stock 540. Journal of General Microbiology 23:243–252
    [Google Scholar]
  4. Beale G. H., Jurand A., Preer J. R. 1969; The classes of endosymbiont of Paramecium aurelia . Journal of Cell Science 5:65–91
    [Google Scholar]
  5. Chao P. K. 1953; Kappa concentrations per cell in relation to the lifecycle, genotype and mating type in Paramecium aurelia, variety 4. Proceedings of the National Academy of Sciences of the United States of America 39:103–113
    [Google Scholar]
  6. Gibson I. 1965; The replication of metagons and mu particles from Paramecium in another cell-Didinium. Genetical Research 6:398–410
    [Google Scholar]
  7. Jurand A., Preer L. B. 1969; Ultrastructure of flagellated lambda symbionts in Paramecium aurelia . Journal of General Microbiology 54:359–364
    [Google Scholar]
  8. Kung C. 1968 Oxidative metabolism of kappa particles from Paramecium aurelia stock 51 in relation to their nature and origin Ph.D. Thesis University of Pennsylvania:
    [Google Scholar]
  9. Kung C. 1970; The electron transport system of kappa particles from Paramecium aureliastock 51. Journal of General Microbiology 61:371–378
    [Google Scholar]
  10. Preer J. R. 1968; Genetics of the protozoa. In Research in Protozoology 3 pp. 139–288 Chen T. Edited by Oxford: Pergamon Press;
    [Google Scholar]
  11. Ridley S. M., Leech R. M. 1970; Division of chloroplasts in an artificial environment. Nature; London: 227463–465
    [Google Scholar]
  12. Rowe E. A., Gibson I., Cavill A. 1971; The effects OF growth conditions on the esterases of Paramecium aurelia . Biochemical Genetics 5:151–159
    [Google Scholar]
  13. Smith-Sonneborn J. E., van Wagtendonk W. J. 1964; Purification and chemical characterization of kappa of stock 51, Paramecium aurelia . Experimental Cell Research 33:50–59
    [Google Scholar]
  14. Soldo A. T. 1960; Cultivation of two strains of killer Paramecium aurelia in axenic medium. Proceedings of the Society for Experimental Biology and Medicine 105:612–615
    [Google Scholar]
  15. Soldo A. T. 1963; Axenic culture of Paramecium-some observation on the growth behaviour and nutritional requirements of a particle bearing strain of Paramecium aurelia 299. Annals of the New York Academy of Sciences 108:380–388
    [Google Scholar]
  16. Soldo A. T., Godoy G. A., van Wagtendonk W. J. 1966; Growth of particle-bearing and particle-free Paramecium aurelia in axenic culture. Journal of Protozoology 13:492–497
    [Google Scholar]
  17. Soldo A. T., van Wagtendonk W. J., Godoy G. A. 1970; Nucleic acid and protein content of purified endosymbiont particles of Paramecium aurelia . Biochemica et biophysica acta 204:325–333
    [Google Scholar]
  18. Sonneborn T. M. 1959; Kappa and related particles in Paramecium. Advances in Virus Research 6:229–356
    [Google Scholar]
  19. Sonneborn T. M. 1965; The metagon, RNA and cytoplasmic inheritance. American Naturalist 99:279–307
    [Google Scholar]
  20. Stevenson I. 1967; Diaminopimelic acid in the mu particles of Paramecium aurelia . Nature; London: 215434–435
    [Google Scholar]
  21. Stevenson I. 1969; The biochemical status of mu particles in Paramecium aurelia . Journal of General Microbiology 57:61–75
    [Google Scholar]
  22. van Wagtendonk W. J., Clark J. A. D., Godoy G. A. 1963; The biological status of lambda and related particles in Paramecium aurelia . Proceedings of the National Academy of Sciences of the United States of America 50:835–838
    [Google Scholar]
  23. van Wagtendonk W. J., Goldman P. H., Smith W. L. 1970; The axenic culture of strains of the various syngens of Paramecium aurelia . Journal of Protozoology 17:389–391
    [Google Scholar]
  24. van Wagtendonk W. J., Tanguay R. B. 1963; The chemical composition of lambda in Paramecium aurelia, stock 299. Journal of General Microbiology 33:395–400
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-68-3-253
Loading
/content/journal/micro/10.1099/00221287-68-3-253
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error