Summary: Haploid recombinant selection has been used to map the loci of 24 auxotrophic mutations on a circular linkage group in an industrial strain of The features of crosses are similar to those in A3(2), except for the recovery of variable numbers of heterokaryons on selective media incrosses. By a suitable choice of selected marker combinations, heterokaryons can be largely eliminated. The linkage map ofis extremely similar to that ofA 3(2); this may indicate a conservation of gene arrangement in pro-karyotes as already suggested by the similarity of linkage relationships inand Salmonella typhimurium.S. rimosus map provides further support for the idea of circular symmetry of the Streptomyces linkage map first proposed forA 3(2).


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