1887

Abstract

SUMMARY: Mycoplasma strain y grew well in a partly defined medium containing charcoal- treated bovine serum albumin (BSA) with added elaidate, elaidate oleate, or elaidate a saturated fatty acid containing 10 to 15, 22 or 24 carbon atoms. With elaidate a saturated acid containing 16 to 20 carbon atoms there was good growth after a variable lag period. When elaidate was the only fatty acid supplied it comprised from 93 to 95 % of the total fatty acids in stationary-phase myco- plasmas, the remainder being derived from contaminating fatty acids in the medium. There was no isomerization of elaidate to oleate. When grown with elaidate a shorter-chain saturated acid (C, C) or with a long-chain acid (C, C), elaidate was incorporated preferentially during the early stages of growth, increasing proportions of the saturated acids being incorporated during the later stages. When grown with elaidate a saturated acid of intermediate chain length (C to C), or with elaidate oleate, both fatty acids were incorporated in approximately equal proportions throughout growth. The preferential incorporation of elaidate in the early growth stages with some pairs of fatty acids is explained by a competition between the two fatty acids for the BSA binding sites.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-68-2-167
1971-10-01
2024-04-23
Loading full text...

Full text loading...

/deliver/fulltext/micro/68/2/mic-68-2-167.html?itemId=/content/journal/micro/10.1099/00221287-68-2-167&mimeType=html&fmt=ahah

References

  1. Cottew G. S. 1970; Mycoplasmas isolated from cattle in Australia. Australian Veterinary Journal 46:378–381
    [Google Scholar]
  2. Kopaczyk K. C., Radin N. S. 1965; In vivo conversions of cerebroside and ceramide in rat brain. Journal of Lipid Research 6:140–145
    [Google Scholar]
  3. McElhaney R. N., Tourtellotte M. E. 1970; The relationship between fatty acid structure and the positional distribution of esterified fatty acids in phosphatidyl glycerol from Mycoplasma laidlawii b. Biochimica et biophysica acta 202:120–128
    [Google Scholar]
  4. Morris L. J. 1964; Specific separations by chromatography on impregnated adsorbents. In New Biochemical Separations pp. 295–319 James A. T., Morris L. J. Edited by London: Van Nostrand;
    [Google Scholar]
  5. Plackett P., Rodwell A. W. 1970; Glycerolipid biosynthesis by Mycoplasma strain y. Biochimica et biophysica acta 210:230–240
    [Google Scholar]
  6. Rodwell A. W. 1967; The nutrition and metabolism of Mycoplasma: progress and problems. Annals of the New York Academy of Sciences 143:88–109
    [Google Scholar]
  7. Rodwell A. W. 1968; Fatty-acid composition of Mycoplasma lipids: biomembrane with only one fatty acid. Science; New York: 1601350–1351
    [Google Scholar]
  8. Rodwell A. W. 1969; The supply of cholesterol and fatty acids for the growth of mycoplasmas. Journal of General Microbiology 58:29–37
    [Google Scholar]
  9. Spector A. A., John K., Fletcher J. E. 1969; Binding of long-chain fatty acids to bovine serum albumin. Journal of Lipid Research 10:56–67
    [Google Scholar]
  10. Spector A. A., Steinberg D., Tanaka A. 1965; Uptake of free fatty acids by Ehrlich ascites tumor cells. Journal of Biological Chemistry 240:1032–1041
    [Google Scholar]
  11. Turner A. W., Campbell A. D., Dick A. T. 1935; Recent work on pleuropneumonia Contagiosa boum in North Queensland. Australian Veterinary Journal 11:63–71
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-68-2-167
Loading
/content/journal/micro/10.1099/00221287-68-2-167
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error