@article{mbs:/content/journal/micro/10.1099/00221287-68-1-65, author = "Kurowski, W. M. and Pirt, S. J.", title = "The Iron Requirement of Agrobacterium tumefaciens for Growth and 3-Ketosucrose Production. The Removal of Iron from Solutions by Seitz Filters", journal= "Microbiology", year = "1971", volume = "68", number = "1", pages = "65-69", doi = "https://doi.org/10.1099/00221287-68-1-65", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-68-1-65", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", abstract = "SUMMARY: Medium for growth of Agrobacterium tumefaciens was made iron-deficient by passage through Seitz filter pads, which removed up to 3·2 mg. Fe3+ from aqueous FeCl3 solutions. The growth rate of A. tumefaciens in this medium was retarded, no 3-ketosucrose accumulated in the culture supernatant and no glucoside 3-dehydrogenase activity was detected in the cells. Addition of Fe3+ to the filtered medium restored the growth rate, glucoside 3-dehydrogenase activity and 3-ketosucrose production. The optimum iron concentrations for maximum glucoside 3-dehydrogenase activity (1 to 3 mg. Fe2+/l.) and yield of 3-ketosucrose (0·5 mg. Fe2+/l.) were considerably less than the minimum requirement of 15 mg. Fe2+/1. for the maximum growth rate.", }