1887

Abstract

SUMMARY: Carbohydrate fermentation by the flagellate protozoan results in the production of large amounts of hydrogen by an unknown mechanism. Impermeability of to pyruvate at neutral pH may explain earlier failures of other workers to demonstrate unequivocally the intermediate participation of pyruvate in this fermentation. Intact cells of have now been shown to ferment pyruvate at pH 4·0. In addition pyruvate is metabolized rapidly at pH 6·0 by cell-free extracts although not by intact cells. Extracts of have been found to convert pyruvate to equimolar amounts of acetate, H and CO. Formate was not metabolized by either intact cells or extracts, although an active hydrogenase was present. It is concluded that this protozoan possesses a mechanism of hydrogen formation similar to that found in the saccharolytic clostridial bacteria.

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/content/journal/micro/10.1099/00221287-68-1-27
1971-09-01
2021-07-31
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