SUMMARY: Staphylococci incubated with calf thymus histone F2 A or teat canal cationic protein bound more I bovine serum albumin than untreated bacteria. In experiments a similar increased binding of labelled albumin was observed in bacteria deposited in the teat canal of the cow and recovered 24 h. later. Antimicrobial cationic proteins may become attached to the surface of bacteria deposited in the teat canal. Incubation of staphylococci, containing C-labelled protoplasmic proteins, in isotonic saline with calf thymus histone F2A or teat canal cationic proteins caused a rapid release of the labelled protein followed by a slower release which continued for 5 h. Bovine serum albumin induced no greater protein leakage than isotonic saline.


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