1887

Abstract

SUMMARY: Attempts to demonstrate the production of toxin by pathogenic strains of have proved negative; a variety of growth conditions and methods of organism extraction were used, several of which were previously described by other workers as yielding toxins. Toxin production appears to be a strain characteristic and not a property of all pathogenic strains. Acid phosphatases with pH optima at 3·6 and 5·6, a peptidase with optimum pH 6·6 and -glucosidase have been isolated from both blastospore and mycelial forms of and their properties examined. The occurrence in organism extracts of alkaline phosphatase and peptidase (optimum pH 3·6) has been confirmed; no activity of mucopolysaccharase, neuraminidase, -glucuronidase or phospholipase A and B could be demonstrated. The possible role of hydrolytic enzymes in pathogenicity is discussed.

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/content/journal/micro/10.1099/00221287-67-3-255
1971-08-01
2021-10-22
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References

  1. Augustyn J. M., Elliott W. B. 1969; A modified hydroxamate assay of phospholipase A activity. Analytical Biochemistry 31:246–250
    [Google Scholar]
  2. Bartels W., Christopherson J. 1970; Einige Eigenschaften der Alkalischen Phosphatase von Candida albicans. Hoppe-Seyler’s Zeitschrift für physiologische Chemie 351:61–67
    [Google Scholar]
  3. Chattaway F. W., Holmes M. R., Barlow A. J. E. 1968; Cell wall composition of mycelial and blastospore forms of Candida albicans. Journal of General Microbiology 51:367–376
    [Google Scholar]
  4. Dawson R. M. C. 1956; Liver glycerylphosphorylcholine diesterase. Biochemical Journal 62:689–693
    [Google Scholar]
  5. De Palma P. A. 1966 Biochemical and structural variations in the cell wall of Candida albicans under conditions of controlled morphogenesis. Thesis, Department of Botany, Boston University; Boston, Mass:
    [Google Scholar]
  6. Duerksen J. D., Halvorson H. 1958; Purification and properties of an inducible β-glucosidase of yeast. Journal of Biological Chemistry 233:1113–1120
    [Google Scholar]
  7. Fishman W. H. 1963; β-Glucuronidase. In Methods of Enzymatic Analysis pp. 869–874 Edited by Bergmeyer H.-U. New York: Academic Press;
    [Google Scholar]
  8. Fiske C. H., Subbarow Y. 1925; The colorimetric determination of phosphorus. Journal of Biological Chemistry 66:375–400
    [Google Scholar]
  9. Hanahan D. J. 1952; The enzymatic degradation of phosphatidyl choline in diethyl ether. Journal of Biological Chemistry 195:199–206
    [Google Scholar]
  10. Hurley R., Stanley V. C. 1969; Cytopathic effects of pathogenic and non-pathogenic species of Candida on cultured mouse epithelial cells: relation to the growth rate and morphology of the fungi. Journal of Medical Microbiology 2:63–74
    [Google Scholar]
  11. Isenberg H. D., Allerhand J., Berkman J. I. 1963; An endotoxin-like fraction extracted from the cells of C. albicans. Nature, London 197:516–517
    [Google Scholar]
  12. Iwata K., Uchida K. 1967a; ‘Canditoxin’ 2. Isolation and purification of the toxic substance. Medicine and Biology (Tokyo) 74:351–356
    [Google Scholar]
  13. Iwata K., Uchida K. 1967b; ‘Canditoxin’ 3. Repurification of the toxic substance and physicochemical properties of the purified fraction. Medicine and Biology (Tokyo) 75:192–195
    [Google Scholar]
  14. Iwata K., Uchida K. 1968; ‘Canditoxin’ 5. Enzymatic activity, with special reference to phosphatase. Medicine and Biology (Tokyo) 77:159–164
    [Google Scholar]
  15. Iwata K., Uchida K., Endo H. 1967; ‘Canditoxin’, a new toxic substance isolated from a strain of Candida albicans. I. Relationship between strains and virulence and conditions for the toxic substance production. Medicine and Biology (Tokyo) 74:345–355
    [Google Scholar]
  16. Kim Y. P., Adachi K., Chow D. 1962; Leucine aminopeptidase in Candida albicans. Journal of Investigative Dermatology 38:115–116
    [Google Scholar]
  17. Kobayashi G. S., Friedman L. 1964; Characterization of the pyrogenicity of Candida albicans, Saccharomyces cerevisiae and Cryptococcus neoformans. Journal of Bacteriology 88:660–666
    [Google Scholar]
  18. Kobayashi G. S., Friedman L., Kofroth J. F. 1964; Some cytological and pathogenic properties of spheroplasts of Candida albicans. Journal of Bacteriology 88:795–801
    [Google Scholar]
  19. Kurup P. V. 1963; Histochemical demonstration of alkaline phosphatase in Candida. Current Science 32:408–410
    [Google Scholar]
  20. Linhardt K., Walter K. 1963; Phosphatases. In Methods of Enzymatic Analysis pp. 783–785 Edited by Bergmeyer H.-U. New York: Academic Press;
    [Google Scholar]
  21. Louria D. B., Brayton R. G., Finkel G. 1963; Studies on the pathogenesis of experimental Candida albicans infections in mice. Sabouraudia 2:271–283
    [Google Scholar]
  22. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  23. Mahadevan S., Nduaguba J. C., Tappel A. L. 1967; Sialidase of rat liver and kidney. Journal of Biological Chemistry 242:4409–4413
    [Google Scholar]
  24. Mankowski Z. T. 1968; Production of glycoprotein by Candida albicans in a synthetic medium and its influence on the growth of newborn mice. Mycopathologica et mycologica applicata 34:113–118
    [Google Scholar]
  25. Masler L., Šikl D., Bauer S., Sandula J. 1966; Extracellular polysaccharide-protein complexes produced by selected strains of Candida albicans (Robin) Berkhout. Folia microbiologica, Praha 11:373–378
    [Google Scholar]
  26. Mathews M. B. 1966; Animal mucopolysaccharidases. In Methods in Enzymology 8 pp. 654–662 Edited by Neufeld E. F., Ginsburg V. New York: Academic Press;
    [Google Scholar]
  27. Mourad S., Friedman L. 1961; Pathogenicity of Candida. Journal of Bacteriology 81:550–556
    [Google Scholar]
  28. Remold H., Fasold H., Staib F. 1968; Purification and characterization of a proteolytic enzyme from Candida albicans. Biochimica et biophysica acta 167:399–406
    [Google Scholar]
  29. Sandula J., Kocková-Kratochvílová A., Zamecnikova A. 1963; Genus Candida Berkhout. II. Pathogenicity of the species C. albicans (Robin) Berkhout. Folia microbiologica, Praha 8:313–317
    [Google Scholar]
  30. Seelig M. N. 1966; Mechanisms by which antibiotics increase the incidence and severity of candidiasis and alter the immunological defences. Bacteriological Reviews 30:442–459
    [Google Scholar]
  31. Staib F. 1965; Serum proteins as nitrogen source for yeastlike fungi. Sabouraudia 4:187–193
    [Google Scholar]
  32. Zaikina N. A., Elinov N. P. 1968; Fungal plasmocoagulase. Mycopathologica et mycologia applicata 35:10–16
    [Google Scholar]
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