1887

Abstract

The structure and occurrence of pili on were investigated with the electron microscope. Piliation depended on the growth of the organism. The number and length of pili reached a maximum in the logarithmic phase and then declined during the stationary phase. A change in colonial morphology from a dry to a moist colony was correlated with the decline from maximum piliation. The distribution of pili as mono- or bipolar was not a function of the growth phase of the culture. Moreover, the number of pili per cell was not uniform within the culture during the growth cycle. Pili, observed by negative staining, had mean diameters of 6·0±2·8 nm. The wide variation of diameter on individual filament was thought to be due to the flexible nature of the pili. This interpretation was supported by the observation that filaments were capable of extreme coiling and bending. When negatively stained the pili did not appear hollow and seemed to differ from the rigid tube-like structure of type I pili (observed in ) by being flexible and rod-like.

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/content/journal/micro/10.1099/00221287-67-2-135
1971-08-01
2022-01-23
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References

  1. Bradley D. E. 1965; The morphology and physiology of bacteriophages as revealed by the electron microscope. Journal of the Royal Microscopical Society 84:257–316
    [Google Scholar]
  2. Bradley D. E. 1966; The structure and infective process of a Pseudomonas aeruginosa bacteriophage containing ribonucleic acid. Journal of General Microbiology 45:83–96
    [Google Scholar]
  3. Brinton C. C. Jun 1965; The structure, function, synthesis and genetic control of bacterial pili and a molecular model for DNA and RNA transport in Gram-negative bacteria. Transactions of the New York Academy of Sciences 21:1003–1054
    [Google Scholar]
  4. Fuerst J. A., Hayward A. C. 1969; Surface appendages similar to fimbriae (pili) on Pseudomonas species. Journal of General Microbiology 58:227–237
    [Google Scholar]
  5. Heumann W., Marx R. 1964; Feinstruktur und Funktion der Fimbrien bei dem stembildenden Bakterium Pseudomonas echinoides . Archiv für Mikrobiologie 47:325–337
    [Google Scholar]
  6. Houwink A. L., van Iterson W. 1950; Electron microscopal observations on bacterial cytology. II. A study on flagellation. Biochimica et biophysica acta 5:10–44
    [Google Scholar]
  7. Luft J. H. 1961; Improvements in epoxy resin embedding methods. Journal of Biophysical and Biochemical Cytology 9:409–414
    [Google Scholar]
  8. Marx R., Heumann W. 1962; Über Geisselfeinstrukturen und Fimbrien bei zwei Pseudomonas-Stämmen. Archiv für Mikrobiologie 43:245–254
    [Google Scholar]
  9. Reynolds E. S. 1963; The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. Journal of Cell Biology 77:208–212
    [Google Scholar]
  10. Ryter A., Kellenberger E. 1958; Étude au microscope electronique de plasmas contenant de l’acide deoxyribonucleique. Zeitschrift für Naturforschung 133:597–605
    [Google Scholar]
  11. Tweedy J. M., Park R. W. A. 1968; Evidence for the presence of fimbriae (pili) on Vibrio species. Journal of General Microbiology 51:235–244
    [Google Scholar]
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