1887

Abstract

The structure and occurrence of pili on were investigated with the electron microscope. Piliation depended on the growth of the organism. The number and length of pili reached a maximum in the logarithmic phase and then declined during the stationary phase. A change in colonial morphology from a dry to a moist colony was correlated with the decline from maximum piliation. The distribution of pili as mono- or bipolar was not a function of the growth phase of the culture. Moreover, the number of pili per cell was not uniform within the culture during the growth cycle. Pili, observed by negative staining, had mean diameters of 6·0±2·8 nm. The wide variation of diameter on individual filament was thought to be due to the flexible nature of the pili. This interpretation was supported by the observation that filaments were capable of extreme coiling and bending. When negatively stained the pili did not appear hollow and seemed to differ from the rigid tube-like structure of type I pili (observed in ) by being flexible and rod-like.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-67-2-135
1971-08-01
2019-10-20
Loading full text...

Full text loading...

http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-67-2-135
Loading
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error