Summary: The isolation and properties of mutants of deficient in host specificity functions is described. Two mutant types have been isolated, one deficient in restriction () and another one deficient in both restriction and modification (). These non-restricting mutants are good recipients for plasmid DNA in conjugation experiments with donor strains. Transfer of chromosomal markers was observed in crosses between Hfr strains and non-restricting mutants of , and in crosses between Hfr or Hfr strains and non-restricting recipients. Recombinants from these crosses are unstable, partially diploid strains.


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