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Summary: Partially purified histidinol dehydrogenases from a variety of micro-organisms have been tested with antibodies prepared in rabbits to pure histidinol dehydrogenases (HD enzymes) from Neurospora crassa (NHD) and Salmonella typhimurium (SHD). Cross-reactions using the double diffusion technique were demonstrated only between the pairs N. crassa and Aspergillus nidulans and S. typhimurium and Escherichia coli. The dehydrogenation of histidinol by S. typhimurium and N. crassa was inhibited by their respective antibodies and antibody to NHD inhibited HD enzymes from A. nidulans, Penicillium chrysogenum, Chlorellapyrenoides and spinach in decreasing effectiveness. Antibody to SHD inhibited HD from S. typhimurium, E. coli, and Micrococcus lysodeikticus to approximately the same degree and from Bacillus subtilis to a lesser degree. HD activity from Pseudomonas aeruginosa, Saccharomyces cerevisiae, Schizosaccharomyces pombe, Arthrobacter histidinolovorans and Euglena gracilis was affected by neither of the antibodies.
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