Summary: Extracts of sp. NCIB 9816, grown on naphthalene as sole carbon source, oxidized a number of polycyclic aromatic hydrocarbons in the presence of NADH. The ability of the extracts to oxidize naphthalene in the presence of either NADH or NADPH was rapidly destroyed in air and upon dilution or dialysis but was restored by various reducing agents, in particular sodium borohydride and dithioerythritol. Because of its instability only a twofold purification of naphthalene oxygenase was obtained. On the basis of its behaviour it is proposed that naphthalene oxygenase is an NAD(P)H-dependent dioxygenase with tightly bound Fe ions.


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