1887

Abstract

Summary: Extracts of sp. NCIB 9816, grown on naphthalene as sole carbon source, oxidized a number of polycyclic aromatic hydrocarbons in the presence of NADH. The ability of the extracts to oxidize naphthalene in the presence of either NADH or NADPH was rapidly destroyed in air and upon dilution or dialysis but was restored by various reducing agents, in particular sodium borohydride and dithioerythritol. Because of its instability only a twofold purification of naphthalene oxygenase was obtained. On the basis of its behaviour it is proposed that naphthalene oxygenase is an NAD(P) H-dependent dioxygenase with tightly bound Fe ions.

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/content/journal/micro/10.1099/00221287-67-1-117
1971-07-01
2021-10-26
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References

  1. Catterall F. A., Murray K., Williams P. A. 1971; The configuration of the 1,2-dihydroxy-1,2-dihydronaphthalene formed in the bacterial metabolism of naphthalene. Biochimica et biophysica acta 237:361–363
    [Google Scholar]
  2. Evans W. C., Fernley H. N., Griffiths E. 1965; Oxidative metabolism of phenanthrene and anthracene by soil pseudomonads. Biochemical Journal 95:819–831
    [Google Scholar]
  3. Gibson D. T., Koch J. R., Kallio R. E. 1968; Oxidative degradation of aromatic hydrocarbons by micro-organisms. I. Enzymic formation of catechol from benzene. Biochemistry 7:2653–2662
    [Google Scholar]
  4. Gibson D. T., Cardini G. E., Maseles F. C., Kallio R. E. 1970; Incorporation of oxygen-18 into benzene by Pseudomonas putida . Biochemistry 9:1631–1635
    [Google Scholar]
  5. Gibson D. T., Hensley M., Yoshioka H., Mabry T. J. 1970; Formation of (+) cis-2,3-dihydroxy-1-methylcyclohexa-4,6-diene from toluene by Pseudomonas putida . Biochemistry 9:1626–1630
    [Google Scholar]
  6. Gornall A. G., Bardawill C. S., David M. M. 1949; Determination of serum proteins by means of the biuret reaction. Journal of Biological Chemistry 177:751–766
    [Google Scholar]
  7. Griffiths E., Evans W. C. 1965; A cell-free perhydroxylase system from soil pseudomonads with activity on aromatic hydrocarbons. Biochemical Journal 95:51 p
    [Google Scholar]
  8. Jerina D. M., Daly J. W., Jeffrey A., Gibson D. T. 1971; Cis-1,2-dihydroxy-1,2-dihydronaphthalene, a bacterial metabolite from naphthalene. Archives of Biochemistry and Biophysics 142:394
    [Google Scholar]
  9. Jerina D. M., Daly J. W., Witkop B., Zaltzman-Nirenberg P., Udenfriend S. 1970; 1,2-Naphthalene oxide as an intermediate in the microsomal hydroxylation of naphthalene. Biochemistry 9:147–156
    [Google Scholar]
  10. Kita H., Miyake Y., Kamimoto M., Senoh S., Yamano T. 1969; Studies on oxygenases. I. Comparative studies on 3,4-dihydroxyphenylacetate-2,3-oxygenase and pyrocatechase by electron spin resonance. Journal of Biochemistry (Tokyo) 66:45–50
    [Google Scholar]
  11. Kojima Y., Itada N., Hayaishi O. 1961; Metapyrocatechase: a new catechol-cleaving enzyme. Journal of Biological Chemistry 236:2223–2228
    [Google Scholar]
  12. Levin O. 1962; Column chromatography of proteins: calcium phosphate. In Methods in Enzymology 5 pp. 27–32 Edited by Colowick S. P., Kaplan N. O. London & New York: Academic Press;
    [Google Scholar]
  13. McKenna E. J., Kallio R. E. 1965; The biology of hydrocarbons. Annual Review of Microbiology 12:183–208
    [Google Scholar]
  14. Mori M., Taniuchi H., Kojima Y., Hayaishi O. 1966; Studies of kynurenate hydroxylase: requirement for iron and flavin nucleotide. Biochimica et biophysica acta 128:535–546
    [Google Scholar]
  15. Nozaki M., Ono K., Nakazawa T., Kotani S., Hayaishi O. 1968; Metapyrocatechase. II. The role of iron and sulphydryl groups. Journal of Biological Chemistry 243:2682–2690
    [Google Scholar]
  16. Warburg O., Christian W. 1941; Isolierung und Kristallisation des Gärungferments Enolase. Biochemische Zeitschrift 310:384–391
    [Google Scholar]
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