1887

Abstract

SUMMARY: A method is described for the isolation of biologically active DNA from strain 13 which could transform auxotrophic mutants of to prototrophy. This DNA preparation did not transform similar mutants of Optimal conditions for transformation were pH 8·5 to 9·5 for the lysate during DNA extraction, DNA concentration of 16 μg./ml. and growth of recipient cells for 5 h. before exposure to DNA.

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/content/journal/micro/10.1099/00221287-66-2-215
1971-05-01
2021-10-24
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References

  1. Adelberg A., Mandel M., Chen G. C. C. 1965; Optical conditions for mutagenesis by N-methyl- N’-nitro-N-nitrosoguanidine in Escherichia coli k12. Biochemical and Biophysical Research Communications 18:788–795
    [Google Scholar]
  2. Avadhani N. G., Mehta B. M., Rege D. V. 1969; Genetic transformation in Escherichia coli. Journal of Molecular Biology 42:413–423
    [Google Scholar]
  3. Burton K. 1956; A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochemical Journal 62:315–323
    [Google Scholar]
  4. Chargaff E., Schulman H. M., Shapiro H. S. 1957; Protoplasts of E. coli as sources and acceptors of deoxypentose nucleic acid: Rehabilitation of a deficient mutant. Nature, London 180:851–852
    [Google Scholar]
  5. Coetzee J. N. 1963; Lysogeny in Proteus rettgeri and the host-range of P. rettgeri and P. hauseri bacteriophages. Society for General Microbiology 31:219–229
    [Google Scholar]
  6. Coetzee J. N., Sacks T. G. 1960; Transduction of streptomycin resistance in Proteus Mirabelis. Society for General Microbiology 23:445–455
    [Google Scholar]
  7. Coetzee J. N., Smit J. A. 1970; Properties of Proteus Mirabelis phage 13vir. Journal of General Virology 9:247–249
    [Google Scholar]
  8. Falkow S., Wolhieter J. A., Citarella R. V., Baron L. S. 1964; Transfer of episomic elements to Proteus. I. Transfer of F-linked chromosomal determinants. Journal of Bacteriology 87:209–219
    [Google Scholar]
  9. Harry G. A., Umbreit W. W. 1947; Differentiation between ribose-3-phosphate and ribose-5-phosphate by means of the orcinol–pentose reaction. Journal of Biological Chemistry 167:369–376
    [Google Scholar]
  10. Kaiser A. D., Hogness D. S. 1960; The transformation of Escherichia coli with deoxyribonucleic acid isolated from bacteriophage λdg. Journal of Molecular Biology 2:392–415
    [Google Scholar]
  11. Kauffman F. 1966 The Bacteriology of Enterobacteriaceae Copenhagen: Einar Munksgaard;
    [Google Scholar]
  12. Lowry O. H., Rosebrough W. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent. Journal of Molecular Biology 193:265–275
    [Google Scholar]
  13. Mehta B. M., Rege D. V., Sreenivasan A. 1962; Transformation to prototrophic condition in Escherichia coli induced by deoxyribonucleic acid. Nature, London 193:296–297
    [Google Scholar]
  14. McCarty M., Taylor H. E., Avery O. T. 1946; Biochemical studies of environment factors essential in transformation of pneumococcal types. Cold Spring Harbor Symposia on Quantitative Biology 11:177–183
    [Google Scholar]
  15. Prozesky O. W. 1967; Arginine synthesis in Proteus Mirabelis. Society for General Microbiology 49:325–334
    [Google Scholar]
  16. Rudchenko O. N. 1968; The overcoming of auxotrophy in Escherichia coli spheroplasts dependent on certain amino acids by means of infection with DNAs from prototrophic strains. Genetika 4:106–112
    [Google Scholar]
  17. van Rensburg A. J. 1969; Infection of spheroplasts with DNA from a Proteus Mirabelis bacteriophage. Journal of General Virology 5:437–441
    [Google Scholar]
  18. van Rensburg A. J. 1970 Studies on Proteus and Providence Spheroplasts, L Forms and Bacteriophage Nucleic Acids M.D. Thesis University of Pretoria; South Africa:
    [Google Scholar]
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