1887

Abstract

SUMMARY: Glucose-limited and oxygen-limited chemostat cultures were agitated at 240, 340, 560 and 780 rev./min. In the oxygen-limited culture the growth yield ranged from 0.2 to 0.25 mg. bacteria/mg. glucose, considerably higher than 0.03 to 0.18 observed when glucose was limiting. This finding suggests that the dissolved oxygen interferes in the metabolism of growing Azotobacter. Aldolase activity, but not that of glyceraldehyde-3-phosphate dehydrogenase, isocitrate dehydrogenase or isocitrate lyase, was proportional to dissolved oxygen concentration in each culture. The carbon balance indicated no products other than the bacteria and carbon dioxide.

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/content/journal/micro/10.1099/00221287-66-2-197
1971-05-01
2022-01-16
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References

  1. Dalton H., Postgate J. R. 1969; Growth and physiology of Azotobacter chroococcum in continuous culture. Society for General Microbiology 56:307–319
    [Google Scholar]
  2. Dilworth M. J. 1962; Oxygen inhibition in Azotobacter vinelandii. Biochimica et biophysica acta 56:127–138
    [Google Scholar]
  3. Dixson G. H., Kornberg H. L. 1959; Assay method for key enzymes of the glyoxylate cycle. Biochemical Journal 72:3 p
    [Google Scholar]
  4. Gornall A. G., Bardawill C. S., David M. M. 1949; Determination of serum proteins by means of the biuret reaction. Journal of Biological Chemistry 177:751–766
    [Google Scholar]
  5. Khmel I. A., Gabinskaya K. N., Ierusalimsky N. D. 1965; Growth and nitrogen fixation of Azotobacter vinelandii under different aeration conditions. Mikrobiologiya 34:689–694
    [Google Scholar]
  6. Kornberg A. 1955; Isocitric dehydrogenase of yeast (TPN). In Methods in Enzymology vol 1 pp 705–707 Edited by Colowick S. P., Kaplan N. O. New York: Academic Press;
    [Google Scholar]
  7. Krebs E. G. 1955; Glyceraldehyde-3-phosphate dehydrogenase from yeast.. In Methods in Enzymology vol 1 pp 407–411 Edited by Colowick S. P., Kaplan N. O. New York: Academic Press;
    [Google Scholar]
  8. Mortenson L. E., Wilson P. W. 1954; Initial stage in the breakdown of carbohydrate by Azotobacter vinelandii. Archives of Biochemistry and Biophysics 53:425–435
    [Google Scholar]
  9. Mortenson L. E., Hamilton P. B., Wilson P. W. 1955; Dissimilation of 6-phosphogluconate by Azotobacter vinelandii. Biochimica et biophysica acta 16:238–244
    [Google Scholar]
  10. Nagai S., Nishizawa Y., Aiba S. 1969; Energetics of growth of Azotobacter vinelandii in a glucose-limited chemostat culture. Society for General Microbiology 59:163–169
    [Google Scholar]
  11. Parker C. A., Scutt P. B. 1960; The effect of oxygen on nitrogen fixation by Azotobacter. Biochimica et biophysica acta 38:230–238
    [Google Scholar]
  12. Stone R. W., Wilson P. W. 1952a; Respiratory activity of cell-free extracts of Azotobacter. Journal of Bacteriology 63:605–617
    [Google Scholar]
  13. Stone R. W., Wilson P. W. 1952b; The effect of oxaloacetate on the oxidation of succinate by Azotobacter extracts. Journal of Bacteriology 63:619–622
    [Google Scholar]
  14. Stone R. W., Wilson P. W. 1952c; The incorporation of acetate in acids of the citric acid cycle by Azotobacter extracts. Journal of Biological Chemistry 196:221–225
    [Google Scholar]
  15. Tschapek M., Giamblagi N. 1955; Nitrogen fixation of Azotobacter in soil—its inhibition by oxygen. Archiv fur Mikrobiologie 21:376–390
    [Google Scholar]
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