SUMMARY: Glucose-limited and oxygen-limited chemostat cultures were agitated at 240, 340, 560 and 780 rev./min. In the oxygen-limited culture the growth yield ranged from 0·2 to 0·25 mg. bacteria/mg. glucose, considerably higher than 0·03 to 0·18 observed when glucose was limiting. This finding suggests that the dissolved oxygen interferes in the metabolism of growing Azotobacter. Aldolase activity, but not that of glyceraldehyde-3-phosphate dehydrogenase, isocitrate dehydrogenase or isocitrate lyase, was proportional to dissolved oxygen concentration in each culture. The carbon balance indicated no products other than the bacteria and carbon dioxide.


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