Diaminopimelic Acid and Lysine Auxotrophs of 8602 Free

Abstract

SUMMARY: Lysine auxotrophs have been isolated from 8602. Three of the mutants were deficient in diaminopimelate decarboxylase and accumulated and -diaminopimelic acid (DAP) but otherwise were indistinguishable from the parent strain. The fourth mutant required lysine for optimal growth, grew slowly on - but not -DAP, and the DAP which accumulated in large amounts was solely the -isomer. This mutant was deficient in diaminopimelate epimerase. No significant differences were detected between its wall composition and that of the parent strain but it was particularly sensitive to carbenicillin.

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/content/journal/micro/10.1099/00221287-66-2-161
1971-05-01
2024-03-28
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References

  1. Anwar R. A., Rov C., Watson R. W. 1963; Isolation and structure of uridine nucleotide-peptides from Aerobacter cloacae NRC492. Canadian Journal of Biochemistry and Physiology 41:1065–1072
    [Google Scholar]
  2. Barnes I. J., Bondi A., Moat A. G. 1969; Biochemical characterization of lysine auxotrophs of Staphylococcus aureus. Journal of Bacteriology 99:169–174
    [Google Scholar]
  3. Bodey G. P., Terrell L. M. 1968; In vitro activity of carbenicillin against Gram-negative bacilli. Journal of Bacteriology 95:1587–1590
    [Google Scholar]
  4. Brammar W. J., McFarlane N. F., Clarke P. H. 1966; The uptake of aliphatic amides by Pseudomonas aeruginosa. Society for General Microbiology 44:303–309
    [Google Scholar]
  5. Davis B. S. 1952; Biosynthetic interrelations of lysine diaminopimelic acid and threonine in mutants of. Escherichia coli Nature; London: 169534–536
    [Google Scholar]
  6. Diringer H., Jusic D. 1966; Über die Bindung der meso- Diaminopimelinsäure in Murein von E. coli. Zeitschrift fur Naturforschung 21: b 603–604
    [Google Scholar]
  7. Fensom A. H., Gray G. W. 1969; The chemical composition of the lipopolysaccharide of Pseudomonas aeruginosa. Biochemical Journal 114:185–196
    [Google Scholar]
  8. Fensom A. H., Meadow P. M. 1970; Evidence for two regions in the polysaccharide moiety of the lipopolysaccharide of Pseudomonas aeruginosa 8602. Febs Letters 9:81–84
    [Google Scholar]
  9. Ghuysen J. M., Tipper D. J., Strominger J. L. 1966; Enzymes that degrade bacterial cell walls.. In Methods in Enzymology vol 8 pp 685–699 Edited by Neufeld E. F., Ginsburg V. New York & London: Academic Press;
    [Google Scholar]
  10. Hancock I. C., Meadow P. M. 1959; The extractable lipids of Pseudomonas aeroginosa. Biochimica et biophysica acta 187:366–379
    [Google Scholar]
  11. Hoare D. S., Work E. 1955; The stereoisomers of α,ε-diaminopimelic acid: Their distribution in nature and behaviour towards certain enzyme preparations. Biochemical Journal 61:562–568
    [Google Scholar]
  12. Hoare D. S., Work E. 1957; The stereoisomers of α,ε-diaminopimelic acid. 2. Their distribution in the bacterial order Actinomycetales and in certain Eubacteriales. Biochemical Journal 65:441–447
    [Google Scholar]
  13. Kelly M., Clarke P. H. 1962; An inducible amidase produced by a strain of Pseudomonas aeruginosa. Society for General Microbiology 27:305–316
    [Google Scholar]
  14. Knox K. W., Cullen J., Work E. 1967; An extracellular lipopolysaccharide-phospholipid-protein complex produced by Escherichia coli grown under lysine-limiting conditions. Biochemical Journal 103:192–201
    [Google Scholar]
  15. Knudsen E. T., Rolinson G. N., Sutherland R. 1967; Carbenicillin: A new semisynthetic penicillin active against Pseudomonas pyocyanea. British Medical Journal iii:75–78
    [Google Scholar]
  16. Leive L., Davis B. D. 1965; The transport of diaminopimelate and cystine in Escherichia coli. Journal of Biological Chemistry 240:4362–4369
    [Google Scholar]
  17. Lilly M. D., Clarke P. H., Meadow P. M. 1963; The accumulation of nucleotides by Escherichia coli strain 26-26. Society for General Microbiology 32:103–116
    [Google Scholar]
  18. Meadow P. M., Work E. 1959; Biosynthesis of diaminopimelic acid and lysine in Escherichia coli. 1. The incorporation of 14C from various organic precursors into the diaminopimelic acid of a lysine-requiring mutant. Biochemical Journal 72:396–400
    [Google Scholar]
  19. Salton M. R. J. 1964 The Bacterial Cell Wall pp 108–109 Amsterdam, Holland: Elsevier;
    [Google Scholar]
  20. Strominger J. L., Threnn R. H. 1959; Accumulation of a uridine nucleotide in Staphylococcus aureus as the consequence of lysine deprivation. Biochimica et biophysica acta 36:83–92
    [Google Scholar]
  21. White P. J., Kelly B., Suffling A., Work E. 1964; Variation of activity of bacterial diaminopimelate decarboxylase under different conditions of growth. Biochemical Journal 91:600–610
    [Google Scholar]
  22. White P. J., Lejeune B., Work E. 1969; Assay and properties of diaminopimelate epimerase from Bacillus megaterium. Biochemical Journal 113:589–601
    [Google Scholar]
  23. Work E. 1957; Reaction of ninhydrin in add solution with straight chain amino acids containing two amino groups and its application to the estimation of a,e-diaminopimelate. Biochemical Journal 67:416–423
    [Google Scholar]
  24. Work E. 1960; The biosynthesis and fate of lysine in bacteria. Colloques Internationaux du Centre National de la Recherche Scientifique 92:143–169
    [Google Scholar]
  25. Work E. 1963; α,ε-Diaminopimelic acid.. In Methods in Enzymology vol 6 pp 624–634 Edited by Colowick S. P., Kaplan N. O. New York & London: Academic Press;
    [Google Scholar]
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