%0 Journal Article %A Hung, C.-Y. %A Wells, K. %T Light and Electron Microscopic Studies of Crozier Development in Pyronema domesticum %D 1971 %J Microbiology, %V 66 %N 1 %P 15-27 %@ 1465-2080 %R https://doi.org/10.1099/00221287-66-1-15 %I Microbiology Society, %X SUMMARY: The ultrastructural features of developing croziers are similar to those described in other ascomycete hyphal elements. The variations in vacuolation during development suggest that vacuoles control the movement of cytoplasm. Conjugate nuclear division occurs in the crozier before segmentation. During this division the continuous spindle fibres of the two nuclei are essentially parallel and extend from polar centriolar plaques. All the observations made with the electron microscope indicate that the nuclear envelope remains intact during division. After conjugate nuclear division the terminal cell is delimited. Subsequently, a septum is formed between the penultimate cell and the stalk cell. Both septa are formed by an inward deposition of wall material and possess a single central pore. The walls between a portion of the stalk and terminal cells dissolve and protoplasmic continuity is established between the two hyphal elements. %U https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-66-1-15