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The number and volume distribution of microbes can be estimated with an electronic particle counter (Kubitschek, 1969). However, when chains of bacteria are present each chain is counted as one large particle. An attempt to overcome this difficulty was made using Streptococcus thermophilus KQ, a chain-forming bacterium, as a test organism. Chains of cells were fixed in formaldehyde and then subjected to ultrasonic treatment to break them down into single units.
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