1887

Abstract

SUMMARY: Mitochondria isolated from Tetrahymena pyriformis carried out oxidative phosphorylation with succinate and 2-oxoglutarate as substrates. Electron transport was inhibited by rotenone, piericidin A, antimycin A and cyanide. Succinate-cytochrome and ferricyanide oxidoreductases were antimycin A sensitive. NADH-cytochrome and ferricyanide oxidoreductases were only partially inhibited by high concentrations of rotenone. Externally added NADH gave no oxygen uptake in the absence of artificial electron acceptors. The mitochondria contained haems and protohaem, and difference spectra revealed the presence of cytochromes and a pigment with an extinction maximum at 620 nm. Steady-state and kinetic measurements of cytochrome components were made. Several kinetically distinct flavoprotein components were present. Kinetic measurements suggested that the reduced 620 nm. component reacted sufficiently rapidly with molecular oxygen to have been the terminal oxidase.

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/content/journal/micro/10.1099/00221287-65-3-359
1971-03-01
2021-10-26
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References

  1. Chance B. 1951; Rapid and sensitive spectrophotometry. III. A double beam apparatus.. Review of Scientific Instruments 22:634–638
    [Google Scholar]
  2. Chance B. 1954; Regeneration and recirculation of reactants in the rapid flow apparatus.. Discussions of the Faraday Society 17:120–128
    [Google Scholar]
  3. Chance B. 1957; Techniques for the assay of the respiratory enzymes.. Methods in Enzymology 4:273–329
    [Google Scholar]
  4. Chance B., Williams G. R. 1956; The respiratory chain and oxidative phosphorylation.. Advances in Enzymology 17:65–134
    [Google Scholar]
  5. Eichel H. J. 1954; Studies on the oxidation of succinic acid by cell-free homogenates of Tetrahymena pyriformis s and w.. Journal of Biological Chemistry 206:159–169
    [Google Scholar]
  6. Evans D. A., Lloyd D. 1966; Mitochondria from Polytomella caeca.. Journal of Protozoology 13:Suppl.119
    [Google Scholar]
  7. Falk J. E. 1964 Porphyrins and Metalloporphyrins New York: Elsevier Publishing Co.;
    [Google Scholar]
  8. Hall C., Wu M., Crane F., Takahashi H., Tamura S., Folkers K. 1966; Piericidin A: a new inhibitor of mitochondrial electron transport.. Biochemical and Biophysical Research Communications 25:373–377
    [Google Scholar]
  9. Kobayashi S. 1965; Preparation and properties of mitochondria from the ciliated protozoon Tetrahymena.. Journal of Biochemistry (Tokyo) 58:444–457
    [Google Scholar]
  10. Lehninger A. L. 1955; Oxidative phosphorylation.. Harvey Lectures 49:176–215
    [Google Scholar]
  11. Lloyd D. 1965; Respiratory control in mitochondria isolated from the colourless algae Prototheca zopfii.. Biochimica et biophysica acta 110:425–426
    [Google Scholar]
  12. Lloyd D., Brightwell R., Venables S. E., Roach G. I., Turner G. 1971; Subcellular fractionation of Tetrahymena pyriformis st by zonal centrifugation: changes in activities and distribution of enzymes during the growth cycle and on starvation.. Journal of General Microbiology 65:209–223
    [Google Scholar]
  13. Lloyd D., Brookman J. S. G. 1967; An oxygen electrode vessel.. Biotechnology and Bioengineering 9:271–272
    [Google Scholar]
  14. Lloyd D., Griffiths A. J. 1968; The isolation of mitochondria from the amoeba Hartmanella castellanii Neff.. Experimented Cell Research 51:291–300
    [Google Scholar]
  15. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent.. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  16. Luft J. H. 1956; Permanganate – a new fixative for electron microscopy.. Journal of Biophysical and Biochemical Cytology 2:799–801
    [Google Scholar]
  17. Mahler H. R. 1955; DPNH-cytochrome c reductase (animal).. Methods in Enzymology 2:688–693
    [Google Scholar]
  18. Ohnishi T., Kawaguchi K., Hagehara B. 1966; Preparation and some properties of yeast mitochondria.. Journal of Biological Chemistry 241:1797–1806
    [Google Scholar]
  19. Parsons D. F. 1966; Ultrastructure and molecular aspects of cell membranes.. Proceedings of the Canadian Cancer Research Conference 7:193–246
    [Google Scholar]
  20. Ryley J. F. 1952; Studies on the metabolism of the protozoa. III. Metabolism of the ciliate, Tetrahymena pyriformis (Glaucoma pyriformis).. Biochemical Journal 52:483–492
    [Google Scholar]
  21. Slater E. C. 1967; Application of inhibitors and uncouplers for a study of oxidative phosphorylation.. Methods in Enzymology 10:48–57
    [Google Scholar]
  22. Suyama Y., Eyer J. 1968; RNA synthesis in isolated mitochondria from Tetrahymena.. Journal of Biological Chemistry 243:320–328
    [Google Scholar]
  23. Suyama Y., Preer J. R. Jun. 1965; Mitochondrial DNA from protozoa.. Genetics 52:1051–1058
    [Google Scholar]
  24. Turner G., Lloyd D., Chance B. 1969; The electron transport chain of Tetrahymena pyriformis strain st.. Biochemical Journal 114:91
    [Google Scholar]
  25. Van DeVijver G. 1966; Studies on the metabolism of Tetrahamena pyriformis gl. II. Some properties of the terminal oxidation.. Enzymologia 31:382–391
    [Google Scholar]
  26. Watson K., Smith J. E. 1967; Oxidative phosphorylation and respiratory control in mitochondria from Aspergillus niger.. Biochemical Journal 104:332–339
    [Google Scholar]
  27. Yamanaka T., Nagata Y., Okunuki K. 1968; Purification and properties of cytochrome c-553 and cytochrome b-560 from Tetrdhymena pyriformis.. Journal of Biochemistry (Tokyo) 63:753–760
    [Google Scholar]
  28. Yang C. C., Legallais V. 1954; A rapid and sensitive recording spectrophotometer for the visible and ultraviolet region.. Review of Scientific Instruments 25:801–807
    [Google Scholar]
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