1887

Abstract

SUMMARY: The activity of acid and alkaline phosphatase in baker's yeast is de-repressed when the organisms are starved of phosphate. Mutants lacking phosphatase activity and mutants with constitutive phosphatase synthesis were isolated. In all of them acid and alkaline phosphatases were affected simultaneously. Nevertheless, characterization of these two enzymes by sensitivity to inhibition by orthophosphate, temperature inactivation, proteolytic digestion and cation dependence showed that they were clearly distinct. It is suggested that these two enzymes share a common genetic and structural component.

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/content/journal/micro/10.1099/00221287-65-3-291
1971-03-01
2024-04-25
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References

  1. Aono H., Otsuji N. 1968; Genetic mapping of regulator gene phoS for alkaline phosphatase in Escherichia coli. . Journal of Bacteriology 95:1182–1183
    [Google Scholar]
  2. Bennun A., Blum J. J. 1966; Properties of the induced acid phosphatase and of the constitutive acid phosphatase of Euglena.. Biochimica et biophysica acta 128:106–123
    [Google Scholar]
  3. Blum J. J. 1965; Observation on the acid phosphatases of Euglena gracilis. . Journal of Cell Biology 24:223–234
    [Google Scholar]
  4. Boer P., Steyn–Parvè E. 1969; Some kinetic aspects of the mechanism of action of an acid phosphatase from baker’s yeast (Saccharomyces cerevisiae).. Biochimica et biophysica acta 171:360–362
    [Google Scholar]
  5. Bracha M., Yagil E. 1969; Genetic mapping of the phoR regulator gene of alkaline phosphatase in Escherichia coli. . Journal of General Microbiology 59:77–81
    [Google Scholar]
  6. Dorn G. L. 1965; Genetic analysis of phosphatases in Aspergillus nidulans. . Genetical Research (Cambridge) 6:13–26
    [Google Scholar]
  7. Dorn G. L. 1968; Purification and characterization of phosphatase I from Aspergillus nidulans. . Journal of Biological Chemistry 243:3500–3506
    [Google Scholar]
  8. Dorn G. L., Rivera W. 1966; Kinetics of fungal growth and phosphatase formation in Aspergillus nidulans. . Journal of Bacteriology 92:1618–1622
    [Google Scholar]
  9. Echols H., Garen A., Garen S., Torriani A. 1961; Genetic control of repression of alkaline phosphatase in Escherichia coli. . Journal of Molecular Biology 3:425–438
    [Google Scholar]
  10. Fan D. P. 1966; Decay of intact messengers in bacteria.. Journal of Molecular Biology 16:164–179
    [Google Scholar]
  11. Garen A., Levinthal C. 1960; A fine-structure genetic and chemical study of the enzyme alkaline phosphatase of E. coli. I. Purification and characterization of akaline phosphatase.. Biochimica et biophysica acta 38:470–483
    [Google Scholar]
  12. Kaneko A. 1968; Studies on two types of alkaline phosphatase in rat liver.. Journal of Biochemistry 64:785–794
    [Google Scholar]
  13. Kitazume Y., Yčas M., Vincent W. S. 1962; Metabolic properties of a ribonucleic acid fraction in yeast.. Proceedings of the National Academy of Sciences of the United States of America 48:265–282
    [Google Scholar]
  14. Lindegren G., Hwang Y. L., Oshima Y., Lindegren C. L. 1965; Genetical mutants induced by ethyl methanesulfonate in Saccharomyces.. Canadian Journal of Genetics and Cytology 7:491–499
    [Google Scholar]
  15. Markham E., Byrne W. J. 1968; Uptake, storage and utilization of phosphate by yeast. III. The behaviour of phosphate-starved yeast.. Journal of the Institute of Brewing 74:374–378
    [Google Scholar]
  16. McLellan W. L., Lampen J. O. 1963; The acid phosphatase of yeast. Localization and secretion by protoplasts.. Biochimica et biophysica acta 67:324–326
    [Google Scholar]
  17. Monod J., Pappenheimer A. M., Bazire G. 1952; La cinétique de la biosynthèse de la β-galactosidase chez E. coli considérée comme fonction de la croissance.. Biochimica et biophysica acta 9:648–660
    [Google Scholar]
  18. Moses V. 1967; The regulatory process in the derepression of enzyme synthesis. Alkaline phosphatase of Bacillus subtilis.. Biochemical Journal 103:650–659
    [Google Scholar]
  19. Neumann H. 1968; Substrate selectivity in the action of alkaline and acid phosphatases.. Journal of Biological Chemistry 243:4671–4676
    [Google Scholar]
  20. Nyc J. F., Kadner R. J., Crocken B. J. 1966; A repressible alkaline phosphatase in Neurospora crassa. . Journal of Biological Chemistry 241:1468–1477
    [Google Scholar]
  21. Plocke D. J., Levinthal C., Vallee B. L. 1962; Alkaline phosphatase of Escherichia coli: A zinc metalloenzyme.. Biochemistry 1:373–378
    [Google Scholar]
  22. Rautanen N., inen V. 1951; On the phosphatase activity of low-phosphorus Torulopsis utilis. . Acta chimica scandinavica 5:1216–1217
    [Google Scholar]
  23. Rothman F., Coleman J. R. 1968; Kinetics of transcription and translation of a repressed gene.. Journal of Molecular Biology 33:527–531
    [Google Scholar]
  24. Schmidt G., Bartsch G., Lamont M. C., Herman T., Liss M. 1963; Acid phosphatase of baker’s yeast: An enzyme of the external cell surface.. Biochemistry 2:126–131
    [Google Scholar]
  25. Schmidt G., Sen. Laskowski M. 1961; Phosphate ester cleavage (Survey).. The Enzymes 5 Edited by Boyer P. D., Lardy H., Myrback K. New York: Academic Press.;
    [Google Scholar]
  26. Shieh T. R., Wodzinski R. J., Ware J. H. 1969; Regulation of the formation of acid phosphatases by inorganic phosphate in Aspergillus ficuum. . Journal of Bacteriology 100:1161–1165
    [Google Scholar]
  27. Simpson R.T., Vallee B. L. 1969; Zinc cobalt alkaline phosphatases.. Annals of the New York Academy of Sciences 166:670–695
    [Google Scholar]
  28. Suomalainen H., Linko M., Oura E. 1960; Changes in the phosphatase activity of baker’s yeast during the growth phase and location of the phosphatases in the yeast cell.. Biochimica et biophysica acta 37:482–490
    [Google Scholar]
  29. Tonino G. J. M., Steyn-Parvé E. P. 1963; Localization of some phosphatases in yeast.. Biochimica et biophysica acta 67:453–469
    [Google Scholar]
  30. Torriani A. 1960; Influence of inorganic phosphate in the formation of phosphatases by Escherichia coli. . Biochimica et biophysica acta 38:460–469
    [Google Scholar]
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