SUMMARY: Growth rates of in batch cultures were controlled by changing aeration rates. Organisms cultured at high aeration rates grew more rapidly and had a greater proportion of lipopolysaccharide (LPS) than had organisms grown at low aeration rates. The LPS isolated from bacteria grown at a rapid rate had higher hexosamine and 3-deoxyoctulosonic acid (KDO) contents and higher galactosamine to glucosamine ratios than LPS from bacteria grown at a slower rate. These results indicated that environmental conditions affected the content and composition of the bacterial LPS. The LPS of organisms from highly aerated cultures had a larger percentage of unsaturated fatty acids than had that from organisms grown under conditions of low aeration, suggesting that oxygen-dependent desaturation of fatty acids had occurred.


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