1887

Abstract

SUMMARY: Growth rates of in batch cultures were controlled by changing aeration rates. Organisms cultured at high aeration rates grew more rapidly and had a greater proportion of lipopolysaccharide (LPS) than had organisms grown at low aeration rates. The LPS isolated from bacteria grown at a rapid rate had higher hexosamine and 3-deoxyoctulosonic acid (KDO) contents and higher galactosamine to glucosamine ratios than LPS from bacteria grown at a slower rate. These results indicated that environmental conditions affected the content and composition of the bacterial LPS. The LPS of organisms from highly aerated cultures had a larger percentage of unsaturated fatty acids than had that from organisms grown under conditions of low aeration, suggesting that oxygen-dependent desaturation of fatty acids had occurred.

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/content/journal/micro/10.1099/00221287-65-2-201
1971-02-01
2024-04-18
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References

  1. Adams G. A. 1971; The chemical composition of a cell-wall lipopolysaccharide from Neisseria sicca.. Canadian Journal of Biochemistry 49:243–250
    [Google Scholar]
  2. Adams G. A., Kates M., Shaw D. H., Yaguchi M. 1968; Studies on the chemical constitution of cell-wall lipopolysaccharides from Neisseria perflava.. Canadian Journal of Biochemistry 46:1175–1184
    [Google Scholar]
  3. Brown C. M., Rose A. H. 1969; Fatty-acid composition of Candida utilis as affected by growth temperature and dissolved-oxygen tension.. Journal of Bacteriology 99:371–378
    [Google Scholar]
  4. Ellwood D. C., Turner W. H., Hunter J. R., Moody G. R. G. 1969; Changes in the cell-wall composition of a strain of Bacillus subtilis grown in a chemostat.. Biochemical Journal 113:14–15
    [Google Scholar]
  5. Ellwood D. C., Tempest D. W. 1969; Control of teichoic acid and teichuronic acid biosynthesis in chemostat cultures of Bacillus subtilis var. niger.. Biochemical Journal III:1–5
    [Google Scholar]
  6. Herbert D. 1961; Microbial reaction to environment.. Symposia of the Society for General Microbiology 11:391–416
    [Google Scholar]
  7. Hough L., Jones J. K. N., Wadman W. H. 1950; Quantitative analysis of mixtures of sugars by the method of partition chromatography. V. Improved methods for the separation and detection of sugars and their methylated derivatives on the paper chromatogram.. Journal of the Chemical Society1702–1706
    [Google Scholar]
  8. Kates M. 1966; Biosynthesis of lipids in micro-organisms.. Annual Review of Microbiology 20:13–44
    [Google Scholar]
  9. Korczynski M. S., Wheat R. 1970; Environmental influence on lipopolysaccharide composition.. Bacteriological Proceedings56
    [Google Scholar]
  10. Luderitz O., Staub A. M., Westphal O. 1966; Immunochemistry of O and Rantigens of Salmonella and related Enterobacteriaceae.. Bacteriological Reviews 30:192–255
    [Google Scholar]
  11. Morgan W. T. J., Elson L. A. 1934; A colorimetric method for the determination of N-acetylglucosamine and N-acetylchondrosamine.. Biochemical Journal 28:988–995
    [Google Scholar]
  12. Shaw M., Ingraham J. L. 1965; Fatty acid composition of Escherichia coli as a possible controlling factor of the minimal growth temperature.. Journal of Bacteriology 90:141–146
    [Google Scholar]
  13. Sykes J., Tempest D. W. 1965; The effect of magnesium and of carbon limitation on the macromolecular organization and metabolic activity of Pseudomonas sp., strain c–1b.. Biochimica et biophysica acta 103:93–108
    [Google Scholar]
  14. Tempest D. W., Dicks J. W., Ellwood D. C. 1968; Influence of growth condition on the concentration of potassium in Bacillus subtilis var. niger and its possible relationship to cellular ribonucleic acid, teichoic acid and teichuronic acid.. Biochemical Journal 106:237–243
    [Google Scholar]
  15. Tempest D. W., Ellwood D. C. 1969; The influence of growth conditions on the composition of some cell-wall components of Aerobacter aerogenes.. Biotechnology and Bioengineering 11:775–783
    [Google Scholar]
  16. Trevelyan W. E., Procter D. P., Harrison J. S. 1950; Detection of sugars on paper chromatograms.. Nature, London 166:444–445
    [Google Scholar]
  17. Weissbach A., Hurwitz J. 1959; The formation of 2-keto-3-deoxyheptonic acid in extracts of Escherichia coli b.. Journal of Biological Chemistry 234:705–709
    [Google Scholar]
  18. Westphal O., deritz O., Bister F. 1952; Über die Extraction von Bakterien mit Phenolwasser.. Zeitschrift für Naturforschung 7B:148–155
    [Google Scholar]
  19. Young F. E. 1965; Variation in the chemical composition of the cell walls of Bacillus subtilis during growth in different media.. Nature, London 207:104–105
    [Google Scholar]
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