SUMMARY: Radioactively labelled coliphage λ DNA is rapidly and irreversibly bound by competent bacteria of certain cultures of The extent of adsorption in the presence of excess cells was always between 30 and 40% of the DNA added. Bound DNA was rather resistant against degradation to acid-soluble products but the biological activity was completely lost after 60 min. incubation. Reisolated phage λ DNA always banded in CsCl gradient centrifugation at a position characteristic for double stranded λ DNA. It sedimented with normal or slightly reduced velocity in sucrose density gradients. Production of λ phage or of infectious phage λ DNA was not observed.


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