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Volume 65, Issue 2

Fate of Bacteriophage λ DNA After Adsorption by Free

Abstract

SUMMARY: Radioactively labelled coliphage λ DNA is rapidly and irreversibly bound by competent bacteria of certain cultures of The extent of adsorption in the presence of excess cells was always between 30 and 40% of the DNA added. Bound DNA was rather resistant against degradation to acid-soluble products but the biological activity was completely lost after 60 min. incubation. Reisolated phage λ DNA always banded in CsCl gradient centrifugation at a position characteristic for double stranded λ DNA. It sedimented with normal or slightly reduced velocity in sucrose density gradients. Production of λ phage or of infectious phage λ DNA was not observed.

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© Society for General Microbiology 1971
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1971-02-01
2024-12-06
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/content/journal/micro/10.1099/00221287-65-2-153
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Fate of Bacteriophage λ DNA After Adsorption by Haemophilus influenzae
Microbiology 65, 153 (1971); https://doi.org/10.1099/00221287-65-2-153
/content/journal/micro/10.1099/00221287-65-2-153
/content/journal/micro/10.1099/00221287-65-2-153
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