1887

Abstract

SUMMARY: M-protein and the opacity factor (OF) were both extracted by Lancefield's method from certain types of group A streptococci. The two proteins could not be separated by fractional precipitation with ammonium sulphate, column chromatography or polyacrylamide gel electrophoresis. The OF appeared to be closely associated with the high molecular weight fraction of the M-antigen, and there is some evidence that the two may form part of the same molecule.

M-protein and the OF were sought in various other extracts and preparations of M-positive and M-negative variants of serotypes which gave a positive serum opacity reaction (SOR). In all preparations from M-positive variants, the two proteins were always present. In M-negative variants, the OF present in the wall-membrane fraction could be extracted only by ‘phage-associated.’ lysin. All other extracts from M-negative variants were SOR-negative and M-negative. In the SOR-positive extract obtained by phage lysin treatment of M-negative variants, no M-antigen could be detected by precipitin tests, though the extracts contained a protein of similar electrophoretic mobility to the precipitin-positive protein in the extracts of the M-positive variants of the same serotype.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-65-1-69
1971-01-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/micro/65/1/mic-65-1-69.html?itemId=/content/journal/micro/10.1099/00221287-65-1-69&mimeType=html&fmt=ahah

References

  1. Brighton W. D. 1966; Methods for the determination of immunity against group A Streptococcus.. Current Research on Group A Streptococcus231–239 Proceedings of a Symposium, Paris, July 1966. Excerpta Medica Foundation 1968.
    [Google Scholar]
  2. Davis B. J. 1964; Disc electrophoresis. II. Method and application to human serum proteins.. Annals of the New York Academy of Sciences 121:404–427
    [Google Scholar]
  3. Fox E. N., Wittner M. K. 1965; The multiple molecular structure of the M proteins of group A streptococci.. Proceedings of the National Academy of Sciences of the United States of America 54:1118–1125
    [Google Scholar]
  4. Fox E. N., Wittner M. K. 1969; New observations on the structure and antigenicity of the M proteins of the group A streptococcus.. Immunochemistry 6:11–24
    [Google Scholar]
  5. Gooder H. 1961; Association of a serum opacity reaction with serological type in Streptococcus pyogenes.. Journal of General Microbiology 25:347–352
    [Google Scholar]
  6. Gooder H., Maxted W. R. 1958; Protoplasts of group A β-haemolytic streptococci.. Nature, London 182:808–809
    [Google Scholar]
  7. Griffith F. 1934; The serological classification of Streptococcus pyogenes.. Journal of Hygiene, Cambridge 34:542–584
    [Google Scholar]
  8. Hill M. J., Wannamaker L. W. 1968; The serum opacity reaction of Streptococcus pyogenes: general properties of the streptococcal factor and of the reaction in aged serum.. Journal of Hygiene, Cambridge 66:37–47
    [Google Scholar]
  9. Lancefield R. C. 1928; The antigenic complex of Streptococcus haemolyticus. I. Demonstration of a type-specific substance in extracts of Streptococcus haemolyticus.. Journal of Experimental Medicine 47:91–103
    [Google Scholar]
  10. Lancefield R. C., Perlmann G. E. 1952; Preparation and properties of type-specific M antigen isolated from a group A, type 1, hemolytic Streptococcus.. Journal of Experimental Medicine 96:71–82
    [Google Scholar]
  11. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent.. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  12. Michel M. F., Gooder H. 1962; Amino acids, amino sugars and sugars present in the cell walls of some strains of Streptococcus pyogenes.. Journal of General Microbiology 29:199–205
    [Google Scholar]
  13. Swift H. F., Wilson A. T., Lancefield R. C. 1943; Typing group A hemolytic streptococci by M precipitin rections in capillary pipettes.. Journal of Experimental Medicine 78:127–133
    [Google Scholar]
  14. Top F. H., Wannamaker L. W. 1968a; The serum opacity reaction of Streptococcus pyogenes. Frequency of production of streptococcal lipoproteinase by strains of different serological types and relationship to M-protein production.. Journal of Hygiene, Cambridge 66:49–58
    [Google Scholar]
  15. Top F. H., Wannamaker L. W. 1968b; The serum opacity reaction of Streptococcus pyogenes. The demonstration of multiple strain specific lipoproteinase antigens.. Journal of Experimental Medicine 127:1013–1034
    [Google Scholar]
  16. Widdowson J. P., Maxted W. R., Grant D. L. 1970; The production of opacity in serum by group A streptococci and its relationship with the presence of M antigen.. Journal of General Microbiology 61:343–353
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-65-1-69
Loading
/content/journal/micro/10.1099/00221287-65-1-69
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error