RT Journal Article SR Electronic(1) A1 Cochrane, Jean C. A1 Rado, T. A. A1 Cochrane, V. W.YR 1971 T1 Synthesis of Macromolecules and Polyribosome Formation in Early Stages of Spore Germination in Fusarium solani JF Microbiology, VO 65 IS 1 SP 45 OP 55 DO https://doi.org/10.1099/00221287-65-1-45 PB Microbiology Society, SN 1465-2080, AB SUMMARY: Net synthesis of protein and RNA in germinating macroconidia of Fusarium solani began at the time of appearance of germ tubes, RNA being formed first; net DNA synthesis began much later. Studies on precursor incorporation indicated that the ungerminated spore, at the time of its removal from the parent mycelium, had a low but real capacity to synthesize both RNA and protein. Leucine incorporation rose very rapidly to a maximum at about 40 min. after harvest; even in the time required for conventional washing and filtration, incorporation capacity increased threefold. Ultra-centrifuge profiles showed polysome peaks after 15 min. incubation; evidence from ribonuclease treatment was consistent with the existence of polysomes in the spore at the time of removal from the mycelium. Neither protein nor RNA synthesis required a complete medium, but DNA was synthesized only in a medium that supported germination., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-65-1-45