1887

Abstract

SUMMARY: Net synthesis of protein and RNA in germinating macroconidia of began at the time of appearance of germ tubes, RNA being formed first; net DNA synthesis began much later. Studies on precursor incorporation indicated that the ungerminated spore, at the time of its removal from the parent mycelium, had a low but real capacity to synthesize both RNA and protein. Leucine incorporation rose very rapidly to a maximum at about 40 min. after harvest; even in the time required for conventional washing and filtration, incorporation capacity increased threefold. Ultra-centrifuge profiles showed polysome peaks after 15 min. incubation; evidence from ribonuclease treatment was consistent with the existence of polysomes in the spore at the time of removal from the mycelium. Neither protein nor RNA synthesis required a complete medium, but DNA was synthesized only in a medium that supported germination.

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/content/journal/micro/10.1099/00221287-65-1-45
1971-01-01
2019-10-13
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