1887

Abstract

SUMMARY: Synchronization of division of was induced in a semi-continuous culture system. A small-scale apparatus designed for the automatic operation of the system is described. The carbon (glucose)-limited defined medium was supplied at intervals of 1 to 4 h. and half of the diluted culture was discharged. After a number of cycles the glucose supplied each time was just sufficient for the doubling of the ‘self-established’ population. A logarithmic loss of viability occurred at the beginning of each cycle in the presence of fresh medium, and it was thought that part of the population underwent unbalanced growth. This phenomenon and the observation that the viable population consisted of two equal parts dividing synchronously but at different times were thought to reflect a post-division unequal competence of the emerging two halves of the population to cope with the active growth induced by the incoming fresh medium.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-65-1-23
1971-01-01
2021-07-28
Loading full text...

Full text loading...

/deliver/fulltext/micro/65/1/mic-65-1-23.html?itemId=/content/journal/micro/10.1099/00221287-65-1-23&mimeType=html&fmt=ahah

References

  1. Anagnostopoulos G. D. 1970; A rapid dilution technique.. Journal of General Microbiology 64:251–252
    [Google Scholar]
  2. Cutler R. G., Evans J. E. 1966; Synchronization of bacteria by a stationary-phase method.. Journal of Bacteriology 91:469–476
    [Google Scholar]
  3. Dawson P. S. S. 1965; Continuous phased growth, with a modified chemostat.. Canadian Journal of Microbiology 11:893–903
    [Google Scholar]
  4. Dawson P. S. S., Kurz W. G. W. 1969; Continuous phased cultures – a technique for growing, analysing and using microbial cells.. Biotechnology and Bioengineering 11:843–851
    [Google Scholar]
  5. Müller J., Dawson P. S. S. 1968; The operational flexibility of the phased culture technique, as observed by changes in the cell cycle of Candida utilis. . Canadian Journal of Microbiology 14:1115–1126
    [Google Scholar]
  6. Nishi A., Okamura S., Yanagita T. 1967; Shift of cell-age distribution pattern in the later phases of Escherichia coli cultures.. Journal of General and Applied Microbiology 13:103–119
    [Google Scholar]
  7. Pirt S. J. 1967; A kinetic study of the mode of growth of surface colonies of bacteria and fungi.. Journal of General Microbiology 47:181–197
    [Google Scholar]
  8. Pirt S. J., Callow D. S. 1964; Continuous flow culture of the ERK and L types of mammalian cells.. Experimental Cell Research 33:413–421
    [Google Scholar]
  9. Schaechter M., e O., Kjeldgaard N. O. 1958; Dependency on medium and temperature of cell size and chemical composition during balanced growth of Salmonella typhimurium. . Journal of General Microbiology 19:592–606
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-65-1-23
Loading
/content/journal/micro/10.1099/00221287-65-1-23
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error