1887

Abstract

SUMMARY: Tryptophan auxotrophs were isolated from strain 655 following mutagenesis with ultraviolet radiation, -methyl-'-nitro--nitrosoguanidine or ethyl methanesulphonate. The mutants were placed in five mutant classes on the basis of auxotrophic response to and accumulations of tryptophan intermediates or derivatives. The tryptophan loci were mapped by comparing relative distances of each locus from two reference loci, and . Relative distances of loci were determined by ratio test analysis of cotransduction data. The tryptophan loci were shown to be contained on a cluster on the genome; the mapping data suggested the gene order: . The genetically distinct regions presumably control two enzymic functions in the conversion of anthranilic acid to 1-(-carboxyphenylamino)-1-deoxyribulose 5-phosphate.

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1970-12-01
2024-04-25
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References

  1. Adelberg E. A., Mandel M., Chen G. C. C. 1965; Optimal conditions for mutagenesis by N-methyl-N’-nitro-N-nitrosoguanidine in Escherichia coli kI2.. Biochemical and Biophysical Research Communications 18:788–795
    [Google Scholar]
  2. Baird-Parker A. C. 1963; A classification of micrococci and staphylococci based on physiological and biochemical tests.. Journal of General Microbiology 30:409–427
    [Google Scholar]
  3. Bonner D. A., De Moss J. A., Mills S. E. 1965; The evolution of an enzyme.. Evolving Genes and Proteins305–318 Edited by Bryson V., Vogel H. J. New York: Academic Press.;
    [Google Scholar]
  4. Blume A. J., Balbinder E. 1966; Tryptophan operon of Salmonella typhimurium. Fine structure analysis by deletion mapping and abortive transduction.. Genetics 53:577–592
    [Google Scholar]
  5. Carlton B. C., Whitt D. D. 1969; The isolation and genetic characterization of mutants of the tryptophan system of Bacillus subtilis.. Genetics 62:445–460
    [Google Scholar]
  6. Davis B. D. 1949; The isolation of biochemically deficient mutants of bacteria by means of penicillin.. Proceedings of the National Academy of Sciences of the United States of America 35:1–10
    [Google Scholar]
  7. Evans J. B. 1965; Current views and problems relating to the taxonomy of the Micrococcaceae.. International Bulletin of Bacterial Nomenclature and Taxonomy 15:111–112
    [Google Scholar]
  8. Evans J. B., Bradford W. L., Niven C. F. 1955; Comments concerning the taxonomy of the genera Micrococcus and Staphylococcus.. International Bulletin of Bacterial Nomenclature and Taxonomy 5:61
    [Google Scholar]
  9. Gunsalus I. C., Gunsalus C. F., Chakrabarty A. M., Sikes S., Crawford I. P. 1968; Fine structure mapping of the tryptophan genes in Pseudomonas putida.. Genetics 60:419–435
    [Google Scholar]
  10. Hartman P. E., Loper J. C., Serman D. 1960; Fine structure mapping by complete transduction between histidine-requiring Salmonella mutants.. Journal of General Microbiology 22:354–368
    [Google Scholar]
  11. Hayes W. 1968 The Genetics of Bacteria and Their Viruses second edition 642 New York: John Wiley and Sons, Inc.;
    [Google Scholar]
  12. Hunter R., De Moss J. A. 1967; Organization of the tryptophan pathway: a phylogenetic study of the fungi.. Journal of Bacteriology 94:1896–1907
    [Google Scholar]
  13. Kloos W. E., Pattee P. A. 1965a; A biochemical characterization of histidine-dependent mutants of Staphylococcus aureus.. Journal of General Microbiology 39:185–194
    [Google Scholar]
  14. Kloos W. E., Pattee P. A. 1965b; Transduction analysis of the histidine region in Staphylococcus aureus.. Journal of General Microbiology 39:195–207
    [Google Scholar]
  15. Kloos W. E., Rose N. E. 1970; Transformation mapping of tryptophan loci in Micrococcus luteus.. Genetics (In the Press).
    [Google Scholar]
  16. Lederberg J. 1950; Isolation and characterization of biochemical mutants of bacteria.. Methods in Medical Research 3:5–22
    [Google Scholar]
  17. Lederberg J., Lederberg E. M. 1952; Replica plating and indirect selection of bacterial mutants.. Journal of Bacteriology 63:399–406
    [Google Scholar]
  18. Loveless A., Howarth S. 1959; Mutation of bacteria at high levels of survival by ethyl methane sulfonate.. Nature, London 184:1780–1782
    [Google Scholar]
  19. Mortimer R. K., Hawthorne D. C. 1966; Genetic mapping in Saccharomyces.. Genetics 53:165–173
    [Google Scholar]
  20. Naylor H. B., Burgi E. 1956; Observation on abortive infection of Micrococcus lysodeikticus with bacteriophage.. Virology 2:577–593
    [Google Scholar]
  21. Pattee P. A., Baldwin J. N. 1961; Transduction of resistance to chloretetracycline and novobiocin in Staphylococcus aureus.. Journal of Bacteriology 82:875
    [Google Scholar]
  22. Ritz H. L., Baldwin J. N. 1962; A transduction analysis of complex loci governing the synthesis of tryptophan by Staphylococcus aureus.. Proceedings of the Society for Experimental Biology and Medicine 110:667–671
    [Google Scholar]
  23. Roberts C. F. 1967; Complementation analysis of the tryptophan pathway in Aspergillus nidulans.. Genetics 53:233–239
    [Google Scholar]
  24. Rosypal S., Rosypalova A., Horejs J. 1966; The classification of micrococci and staphylococci based on their DNA base composition and Adansonian analysis.. Journal of General Microbiology 44:281–292
    [Google Scholar]
  25. Smith O. H. 1967; Structure of the trpC cistron specifying indoleglycerol phosphate synthetase, and its localization in the tryptophan operon of Escherichia coli.. Genetics 57:95–105
    [Google Scholar]
  26. Swanstrom M., Adams M. A. 1951; Agar layer method for production of higher titre phage stocks.. Proceedings of the Society for Experimental Biology and Medicine 78:372–375
    [Google Scholar]
  27. Yanofsky C. 1955; An isotopic study of the conversion of anthranilic acid to indole.. Journal of Biological Chemistry 217:345–354
    [Google Scholar]
  28. Yanofsky C. 1956; The enzymatic conversion of anthranilic acid to indole.. Journal of Biological Chemistry 223:171–184
    [Google Scholar]
  29. Yanofsky C. 1960; 1-(o-carboxyphenylamino)-1-deoxyribulose 5-phosphate, a new intermediate in the biosynthesis of tryptophan.. Journal of Biological Chemistry 235:2051–2057
    [Google Scholar]
  30. Yanofsky C., Lennox E. S. 1959; Transduction and recombination study of linkage relationships among the genes controlling tryptophan synthesis in Escherichia coli.. Virology 8:425–447
    [Google Scholar]
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