@article{mbs:/content/journal/micro/10.1099/00221287-64-2-247, author = "Calleja, G. B.", title = "Flocculation in Schizosaccharomyces pombe", journal= "Microbiology", year = "1970", volume = "64", number = "2", pages = "247-250", doi = "https://doi.org/10.1099/00221287-64-2-247", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-64-2-247", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", abstract = "SUMMARY: Spontaneous formation of macroscopic aggregates of yeast cells is termed flocculation. Many papers have been written on the subject (for reviews see Comrie, 1952, Gilliland, 1957; Jansen, 1958; Rainbow, 1966; Windisch, 1968), but because of the importance of the phenomenon to the brewer most of the studies have been confined to brewer's yeast. An analogous phenomenon in a fission yeast is described here. Schizosaccharomyces pombe ncyc 132 was grown in 125 ml. Erlenmeyer flasks each containing 10 ml. malt-extract broth (Oxoid, 2%, w/v). The inoculum consisted of 5 × 106 stationary-phase yeast cells from a 24 h. static culture in the same medium in a tightly capped McCartney bottle. Cultures were shaken on a rotatory shaker at 150 rev./min. at 32°; the generation time was 120 min.", }