1887

Abstract

SUMMARY: A mutant of was isolated which showed temperature-sensitive synthesis of DNA. The mutant (11 ) increased in cell mass at 38° without concurrent DNA synthesis, resulting in loss of viability and the production of long filaments. DNA synthesis at 38° continues for approximately 40 min. at a gradually decreasing rate giving an increase of DNA in Casamino acid minimal medium of about 55% over that present at the time of the shift. This residual DNA synthesis is not reduced by increasing the temperature to 42°, but can be increased by enriching of the medium in which the organisms are grown before the shift. The effect of high temperature on DNA synthesis in 11 mimics the effect of chloramphenicol, which is known to allow the completion of rounds of replication of DNA without allowing new initiations. The lesion is essentially irreversible and is not corrected by increasing the osmotic pressure of the growth medium. Phage P22 can develop normally in 11 if added at the time of the shift or 30 min. later. Even if the phage is added 3½ h. after the shift substantial multiplication occurs. Cell division continues for several hours after the temperature shift, resulting in the production of DNA-less cells as reported in a separate publication.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-64-2-127
1970-12-01
2021-07-26
Loading full text...

Full text loading...

/deliver/fulltext/micro/64/2/mic-64-2-127.html?itemId=/content/journal/micro/10.1099/00221287-64-2-127&mimeType=html&fmt=ahah

References

  1. Adelberg E. A., Mandel M., Chen G. 1965; Optimal conditions for mutagenesis by N- methyl-N′-nitro-N-nitrosoguanidine in Escherichia coli k12.. Biochemical and Biophysical Research Communications 18:788–795
    [Google Scholar]
  2. Bazill G. W., Retief Y. 1969; Temperature-sensitive DNA synthesis in a mutant of Bacillus subtilis.. Journal of General Microbiology 56:87–97
    [Google Scholar]
  3. Bonhoeffer F. 1966; DNA transfer and DNA synthesis during bacterial conjugation.. Zeitschrift fur Vererbungslehre 98:141–149
    [Google Scholar]
  4. Budman D. R., Pardee A. B. 1967; Thymidine and thymine incorporation into deoxyribonucleic acid—inhibition and repression by uridine of thymidine phosphorylase of E. coli.. Journal of Bacteriology 94:1546–1550
    [Google Scholar]
  5. Cook T. M., Deitz W. H., Goss W. A. 1966; Mechanism of action of nalidixic acid in Escherichia coli. IV. Effects on the stability of cellular constituents.. Journal of Bacteriology 91:774–779
    [Google Scholar]
  6. Davis B. D., Mingioli E. S. 1950; Mutants of Escherichia coli requiring methionine or vitamin B12.. Journal of Bacteriology 60:17–28
    [Google Scholar]
  7. Fangman W. L., Novick A. 1968; Characterization of two bacterial mutants with temperature-sensitive synthesis of DNA.. Genetics 60:1–17
    [Google Scholar]
  8. Gross J. D., Karamata D., Hempstead P. G. 1968; Temperature-sensitive mutants of Bacillus subtilis defective in DNA synthesis.. Cold Spring Harbor Symposia on Quantitative Biology 33:307–312
    [Google Scholar]
  9. Helmstetter C. E., Cooper S. 1968; DNA synthesis during the division cycle of rapidly growing Escherichia coli B/r.. Journal of Molecular Biology 31:507–518
    [Google Scholar]
  10. Hirota Y., Ryter A., Jacob F. 1968; Thermosensitive mutants of E. coli affected in the processes of DNA synthesis and cellular division.. Cold Spring Harbor Symposia on Quantitative Biology 33:677–693
    [Google Scholar]
  11. Inouye M. 1969; Unlinking of cell division from deoxyribonucleic acid replication in a temperature-sensitive deoxyribonucleic acid synthesis mutant of Escherichia coli.. Journal of Bacteriology 99:842–850
    [Google Scholar]
  12. Jacob F., Brenner S., Cuzin F. 1963; On the regulation of DNA replication in bacteria.. Cold Spring Harbor Symposia on Quantitative Biology 28:329–348
    [Google Scholar]
  13. Kuempel P. L. 1969; Temperature-sensitive initiation of chromosome replication in a mutant of Escherichia coli.. Journal of Bacteriology 100:1302–1310
    [Google Scholar]
  14. Lanka E., Schuster H. 1970; Replication of bacteriophage in Escherichia coli mutants thermo-sensitive in DNA synthesis.. Molecular and General Genetics 106:274–285
    [Google Scholar]
  15. Lark C., Lark K. G. 1964; Evidence for two distinct aspects of the mechanism regulating chromosome replication in Escherichia coli.. Journal of Molecular Biology 10:120–136
    [Google Scholar]
  16. Lark K. G., Renger H. 1969; Initiation of DNA replication in Escherichia coli I5t: Chronological dissection of three physiological processes required for initiation.. Journal of Molecular Biology 42:221–235
    [Google Scholar]
  17. Lark K. G., Repko T., Hoffman E. J. 1963; The effect of amino acid deprivation on subsequent deoxyribonucleic acid synthesis.. Biochimica et biophysica acta 76:9–24
    [Google Scholar]
  18. Maaløe O., Hanawalt P. C. 1961; Thymine deficiency and the normal DNA replication cycle.. Journal of Molecular Biology 3:144–155
    [Google Scholar]
  19. Ricard M., Hirota Y. 1969; Effet des sels sur le processus de division cellulaire d’E. coli.. Comptes rendu hebdomadaire des seances de l’Académie des sciences, Paris 268:1335–1338
    [Google Scholar]
  20. Ward C. B., Glaser D. A. 1969; Analysis of the chloramphenicol-sensitive and chloramphenicol-resistant steps in the initiation of DNA synthesis in E. coli B/r.. Proceedings of the National Academy of Sciences of the United States of America 64:905–912
    [Google Scholar]
  21. Yoshikawa H., Sullivan A., Sueoka N. 1964; Sequential replication of the Bacillus subtilis chromosome. III. Regulation of initiation.. Proceedings of the National Academy of Sciences of the United States of America 52:973–980
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-64-2-127
Loading
/content/journal/micro/10.1099/00221287-64-2-127
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error