1887

Abstract

SUMMARY: The enzymes involved in the tricarboxylic acid cycle and some other related reactions were measured quantitatively in cell-free extracts from the obligate autotroph grown anaerobically, and in the facultative autotroph grown under various conditions. The presence of an incomplete tricarboxylic acid cycle in and in (when grown autotrophically on thiosulphate) is described. The cell-free extracts from these two organisms lacked α-ketoglutarate dehydrogenase and succinyl CoA synthetase. The data from grown on succinate, aerobically or anaerobically, and on glutamate aerobically suggest that both the enzymes are repressed during autotrophic growth. Results are further discussed in relation to possible reasons for autotrophy.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-64-1-29
1970-11-01
2022-01-23
Loading full text...

Full text loading...

/deliver/fulltext/micro/64/1/mic-64-1-29.html?itemId=/content/journal/micro/10.1099/00221287-64-1-29&mimeType=html&fmt=ahah

References

  1. Aleem M. I. H. 1968; Mechanism of oxidative phosphorylation in the chemoautotroph Nitro-bacter agilis.. Biochimica et biophysica acta 162:338–347
    [Google Scholar]
  2. Aleem M. I. H. 1969; Generation of reducing power in chemosynthesis. VI. Energy-linked reactions in the chemoautotroph Thiobacillus neapolitanus.. Antonie van Leeuwenhoek 35:379–391
    [Google Scholar]
  3. Aleem M. I. H. 1970; Oxidation of inorganic nitrogen compounds.. Annual Review of Plant Physiology 21:67–90
    [Google Scholar]
  4. Amarisingham C. R., Davies B. D. 1965; Regulation of α-ketoglutarate dehydrogenase formation in Escherichia coli.. Journal of Biological Chemistry 240:3664–3668
    [Google Scholar]
  5. Anfinsen C. B. 1955; Aconitase from pig heart muscle.. Methods in Enzymology 1:695–698
    [Google Scholar]
  6. Bridger W. A., Ramaley R. F., Boyer P. D. P. 1969; Succinyl coenzyme A synthetase from Escherichia coli.. Methods in Enzymology 13:70–75
    [Google Scholar]
  7. Butler R. G., Umbreit W. W. 1969; Reduced nicotinamide adenine dinucleotide oxidase and α-ketoglutaric dehydrogenase activity in Thiobacillus thiooxidans.. Journal of Bacteriology 97:966–967
    [Google Scholar]
  8. Callely A. G., Rigopoulos N., Fuller R. C. 1968; The assimilation of carbon by Choro-pseudomonas ethylium.. Biochemical Journal 106:615–622
    [Google Scholar]
  9. Carr N. G., Lascelles J. 1961; Some enzymatic reactions concerned in the metabolism of aceto-acetyl-coenzyme A in Athiorhodaceae.. Biochemical Journal 80:70–77
    [Google Scholar]
  10. Cooper R. C. 1964; Evidence for the presence of certain tricarboxylic acid cycle enzymes in Thiobacillus thioparus.. Journal of Bacteriology 88:624–629
    [Google Scholar]
  11. Dixon G. H., Kornberg H. L. 1965; Malate synthetase from baker’s yeast.. Methods in Enzymology 5:633–637
    [Google Scholar]
  12. Fuller R. C., Smillie R. M., Sisler E. C., Kornberg H. L. 1961; Carbon metabolism in Chromatium.. Journal of Biological Chemistry 236:2140–2149
    [Google Scholar]
  13. Gornall A. C., Bardawill C. J., David M. M. 1949; Determination of serum proteins by means of the biuret reaction.. Journal of Biological Chemistry 177:751–766
    [Google Scholar]
  14. Hempfling W. P., Vishniac W. 1965; Oxidative phosphorylation in extracts of Thiobacillus X.. Biochemische Zeitschrift 342:272–287
    [Google Scholar]
  15. Hill L. R., Bradshaw R. A. 1969; Fumarase.. Methods in Enzymology 13:91–99
    [Google Scholar]
  16. Hoare D. S., Moore R. B. 1965; Photoassimilation of organic compounds by autotrophic blue-green algae.. Biochimica et biophysica acta 109:622–625
    [Google Scholar]
  17. Hooper A. B. 1969; Biochemical basis of obligate autotrophy in Nitrosomonas europaea.. Journal of Bacteriology 97:776–779
    [Google Scholar]
  18. Johnson E. J., Abraham S. 1969 a; Enzymes of the intermediary carbohydrate metabolism in the obligate autotrophs Thiobacillus thioparus and Thiobacillus neapolitanus.. Journal of Bacteriology 98:962–968
    [Google Scholar]
  19. Johnson E. J., Abraham S. 1969b; Assimilation and metabolism of exogenous organic compounds by the strict autotrophs Thiobacillus thioparus and Thiobacillus neapolitanus.. Journal of Bacteriology 97:1198–1208
    [Google Scholar]
  20. Kaufman S. 1955; α-Ketoglutarate dehydrogenase system and phosphorylating enzyme from heart muscle.. Methods in Enzymology 1:714–722
    [Google Scholar]
  21. Kelly D. P. 1967; The incorporation of acetate by the chemoautotroph Thiobacillus neapolitanus strain c.. Archiv fur Mikrobiologie 58:99–116
    [Google Scholar]
  22. Kiesow L. 1964; On the assimilation of energy from inorganic sources in autotrophic forms of life.. Proceedings of the National Academy of Sciences of the United States of America 52:980–998
    [Google Scholar]
  23. Ochoa S. 1955; Crystalline condensing enzyme from pig heart.. Methods in Enzymology 1:685–694
    [Google Scholar]
  24. Olson J. A. 1959; The purification and properties of yeast isocitric lyase.. Journal of Biological Chemistry 234:5–10
    [Google Scholar]
  25. Pearce J., Leach C. K., Carr N. G. 1969; The incomplete tricarboxylic acid cycle in the blue-green alga Anabaena variabilis.. The Journal of General Microbiology 55:371–378
    [Google Scholar]
  26. Peeters T. L., Aleem M. I. H. 1970; Oxidation of sulphur compounds and electron transport in Thiobacillus denitrificans.. Archiv fur Mikrobiologie 71:319–330
    [Google Scholar]
  27. Sargeant K., Buck P. W., Ford W. S., Yeo R. G. 1966; Anaerobic production of Thiobacillus denitrificans for the enzyme rhodanase.. Applied Microbiology 14:998–1003
    [Google Scholar]
  28. Smith A. J., Hoare D. S. 1968; Acetate assimilation by Nitrobacter agilis in relation to its ‘obligate autotrophy’.. Journal of Bacteriology 95:844–855
    [Google Scholar]
  29. Smith A. J., London J., Stanier R. Y. 1967; Biochemical basis of obligate autotrophy in blue-green algae and thiobacilli.. Journal of Bacteriology 94:972–983
    [Google Scholar]
  30. Sottocasa G. L., Kuylenstierna B., Ernster L., Bergstrand A. 1967; Separation and some enzymatic properties of the inner and outer membranes of rat liver mitochondria.. Methods in Enzymology 10:448–463
    [Google Scholar]
  31. Stern J. R., Coon M. J., DelCampillo A., Scheider M. C. 1956; Enzymes of fatty acid metabolism. IV. Preparation and properties of coenzyme A transferase.. Journal of Biological Chemistry 221:15–31
    [Google Scholar]
  32. Taylor B. F., Hoare D. S. 1969; A new facultative Thiobacillus and a re-evaluation of the heterotrophic potential of Thiobacillus novellus.. Journal of Bacteriology 100:487–497
    [Google Scholar]
  33. Taylor B. F., Hoare D. S., Hoare S. L. 1969; Thiobacillus denitrificans as an obligate chemo-lithotroph.. Bacteriological Proceedings 69:63
    [Google Scholar]
  34. Trudinger P. A., Kelly D. P. 1968; Reduced nicotinamide adenine dinucleotide oxidation by Thiobacillus neapolitanus and Thiobacillus strain c.. Journal of Bacteriology 95:1962–1963
    [Google Scholar]
  35. Veeger C., DerVartanian D. V., Zeylemaker W. P. 1969; Succinate dehydrogenase.. Methods in Enzymology 13:81–90
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-64-1-29
Loading
/content/journal/micro/10.1099/00221287-64-1-29
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error