1887

Abstract

SUMMARY: A comparative study has been made of the influence of growth environment on the properties of the walls of var. organisms, particularly with respect to ability to bind magnesium ions. The medium NaCl concentration and the nature of the growth-limiting substrate, in chemostat cultures, both affected markedly the subsequent affinity and capacity of the bacterial walls for Mg adsorption. These changes correlated with differences in the phosphate content of the walls, which in turn related largely to changes in wall teichoic acid content. Thus it seemed that whenever a constraint to the adsorption of Mg was applied (either by growing the organisms in a Mg- limited environment, or in one containing high concentrations of a competing ion), walls were synthesized that had an increased teichoic acid content and an increased affinity for magnesium ions. These results support the thesis that anionic polymers in the walls of Bacillus organisms (i.e. teichoic acids and teichuronic acids) are involved in cation assimilation.

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/content/journal/micro/10.1099/00221287-63-3-325
1970-11-01
2021-07-31
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References

  1. Archibald A. R., Armstrong J. J., Baddiley J., Hey B. 1961; Teichoic acid and the structure of bacterial cell walls. Nature; London: 191570–572
    [Google Scholar]
  2. Asbell M. A., Eagon R. G. 1966; Role of multivalent cations in the organization, structure and assembly of the cell wall of Pseudomonas aeruginosa . Journal of Bacteriology 92:380–387
    [Google Scholar]
  3. Brown A. D. 1963; The peripheral structures of Gram-negative bacteria. Biochimica et Biophysica Acta 75:425–435
    [Google Scholar]
  4. Brown A. D. 1965; Hydrogen ion titrations of intact and dissolved lipoprotein membranes. Journal of Molecular Biology 12:491–508
    [Google Scholar]
  5. Ellwood D. C. 1970; The wall content and composition of Bacillus subtilis var niger, grown in a chemostat. Biochemical Journal 118:367–373
    [Google Scholar]
  6. Ellwood D. C., Tempest D. W. 1967; Teichoic acid or teichuronic acid in the walls of Bacillus subtilis varniger, grown in a chemostat. Biochemical Journal 104:69P
    [Google Scholar]
  7. Ellwood D. C., Tempest D. W. 1969; Control of teichoic acid and teichuronic acid biosyntheses in chemostat cultures of Bacillus subtilis var niger . Biochemical Journal, Experientia 111:1–5
    [Google Scholar]
  8. Galdiero F. 1968; Hydrogen ion binding of bacterial cell wall. Experientia 24:352–353
    [Google Scholar]
  9. Galdiero F., Lembo M., Tufano M. A. 1968; Affinity of various cations for Staphylococcus aureus cell wall. Experientia 24:34–36
    [Google Scholar]
  10. Gray G. W., Wilkinson S. G. 1965; The effect of ethylenediaminetetra-acetic acid on the cell walls of some Gram-negative bacteria. Journal of General Microbiology 39:385–399
    [Google Scholar]
  11. Herbert D., Phipps P. J., Tempest D. W. 1965; The chemostat: design and instrumentation. Laboratory Practice 14:1150–1161
    [Google Scholar]
  12. James A. M., Brewer J. E. 1968; Non-protein components of the cell surface of Staphylococcus aureus . Biochemical Journal 107:817–821
    [Google Scholar]
  13. Kushner D. J., Onishi H. 1966; Contribution of protein and lipid components to the salt response of envelopes of an extremely halophilic bacterium. Journal of Bacteriology 91:653–660
    [Google Scholar]
  14. Meers J. L., Tempest D. W. 1969; The influence of environment on the synthesis of magnesium-binding sites on Bacillus subtilis var niger cell walls. Journal of General Microbiology, Experientia 55:x
    [Google Scholar]
  15. Strange R. E., Shon M. 1964; Effects of thermal stress on viability and ribonucleic acid of Aerobacter aerogenes in aqueous suspension. Journal of General Microbiology 34:99–114
    [Google Scholar]
  16. Tempest D. W., Ellwood D. C. 1969; The influence of growth condition on the composition of some cell wall components of Aerobacter aerogenes . Biotechnology and Bioengineering 11:775–783
    [Google Scholar]
  17. Tempest D. W., Meers J. L. 1968; The influence of NaCl concentration of the medium on the potassium content of Aerobacter aerogenes and on the interrelationships between potassium, magnesium and ribonucleic acid in the growing bacteria. Journal of General Microbiology 54:319–325
    [Google Scholar]
  18. Tempest D. W., Strange R. E. 1966; Variation in the content and distribution of magnesium, and its influence on survival, in Aerobacter aerogenes grown in a chemostat. Journal of General Microbiology 44:273–279
    [Google Scholar]
  19. Tempest D. W., Dicks J. W., Ellwood D. C. 1968; Influence of growth condition on the con-centration of potassium in Bacillus subtilis var niger and its possible relationship to cellular ribonucleic acid, teichoic acid and teichuronic acid. Biochemical Journal 106:237–243
    [Google Scholar]
  20. Tempest D. W., Dicks J. W., Meers J. L. 1967; Magnesium-limited growth of Bacillus subtilis in pure and mixed cultures in a chemostat. Journal of General Microbiology 49:139–147
    [Google Scholar]
  21. Tempest D. W., Hunter J. R., Sykes J. 1965; Magnesium-limited growth of Aerobacter aerogenes in a chemostat. Journal of General Microbiology 39:355–366
    [Google Scholar]
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