1887

Abstract

SUMMARY: The deoxyribonucleic acid (DNA) base compositions of 12 recently isolated or new species of mycoplasmas fell within the range known for the Mycoplasma group. By DNA-DNA hybridization a new sterol-nonrequiring strain, -743, was unrelated to or , the latter two strains showing moderate cross-reactivity; swine strain B3 appeared to be related to var. From these results and information in the literature, the mycoplasmas have been divided into six provisional subgroups on the basis of physiological characters, DNA base composition and nucleic acid hybridization. Some speculations are made on the significance of heterogeneity among mycoplasmas.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-63-2-249
1970-10-01
2024-12-09
Loading full text...

Full text loading...

/deliver/fulltext/micro/63/2/mic-63-2-249.html?itemId=/content/journal/micro/10.1099/00221287-63-2-249&mimeType=html&fmt=ahah

References

  1. Bak A. L., Black F. T. 1968; DNA base composition of human T strain mycoplasmas. Nature; London: 2191044
    [Google Scholar]
  2. Bak A. L., Black F. T., Christiansen C., Freundt E. A. 1969; Genome size of Mycoplasma DNA. Nature; London: 2241209
    [Google Scholar]
  3. Barile M. F., Del Giudice R. A., Carski T. R., Gibbs C. J., Morris J. A. 1968; Isolation and characterization of Mycoplasma arginini: spec.nov. Proceedings of the Society for Experimental Biology and Medicine 129:489
    [Google Scholar]
  4. Barile M. F., Schimke R. T., Riggs D. B. 1966; Presence of the arginine dihydrolase pathway in Mycoplasma. Journal of Bacteriology 91:189
    [Google Scholar]
  5. Bode H. R., Morowitz H. J. 1967; Size and structure of the Mycoplasma hominis H39 chromosome. Journal of Molecular Biology 23:191
    [Google Scholar]
  6. Chelton E.T.J., Jones A. S., Walker R. T. 1968; The chemical composition of the nucleic acids and the proteins of some Mycoplasma strains. Journal of General Microbiology 50:305
    [Google Scholar]
  7. Cole B. C., Golightly L., Ward J. R. 1967; Characterization of Mycoplasma strains from cats. Journal of Bacteriology 93:1425
    [Google Scholar]
  8. Cottew G. S., Watson W. A., Arisoy F., Erdag O., Buckley L. S. 1968; Differentiation of Mycoplasma agalactiae from other mycoplasmas of sheep and goats. Journal of Comparative Pathology 78:275
    [Google Scholar]
  9. Davidson M., Thomas L. 1968; Mycoplasma in primates. Bacteriological Proceedings p. 79
    [Google Scholar]
  10. Denhardt D. T. 1966; A membrane filter technique for the detection of complementary DNA. Biochemical and Biophysical Research Communications 23:641
    [Google Scholar]
  11. Dinter Z., Danielsson D., Bakos K. 1965; Differentation of porcine Mycoplasma strains. Journal of General Microbiology 41:77
    [Google Scholar]
  12. Edward D.G.FF. 1967; Problems in classification-an introduction. Annals of the New York Academy of Sciences 143:7
    [Google Scholar]
  13. Edward D.G.FF., Freundt E. A. 1969; Proposal for classifying organisms related to Mycoplasma laidlawii in a family Sapromycetaceae, genus Sapromyces, within the Mycoplasmatales. Journal of General Microbiology 57:391
    [Google Scholar]
  14. Edward D.G.FF., Freundt E. A., Chanock R. M., Fabricant J., Hayflick L., Lemcke R. M., Razin S., Somerson N. L., Wittler R. G. 1967; Recommendations on nomenclature of the order Mycoplasmatales. Science; New York: 1551694
    [Google Scholar]
  15. Gillespie D., Spiegelman S. 1965; A quantitative assay for DNA-RNA hybrids with DNA immobilized on a membrane. Journal of Molecular Biology 12:829
    [Google Scholar]
  16. Hale H. H., Helmboldt C. F., Plastridge W. N., Stula E. F. 1962; Bovine mastitis caused by a Mycoplasma species. Cornell Veterinarian 52:582
    [Google Scholar]
  17. Hoyer B. H., King J. R. 1969; Deoxyribonucleic acid sequence losses in a stable streptococcal L-form. Journal of Bacteriology 97:1516
    [Google Scholar]
  18. Hoyer B. H., McCarthy B. J., Bolton E. T. 1964; A molecular approach to the systematics of higher organisms. Science; New York: 144959
    [Google Scholar]
  19. Jain N. C., Jasper D. E., Dellinger J. D. 1967; Cultural characteristics and serological relationships of some mycoplasmas isolated from bovine sources. Journal of General Microbiology 49:401
    [Google Scholar]
  20. Jonas A. M., Barber T. L. 1969; Mycoplasma mycoides var.capri isolated from a goat in Connecticut. Journal of Infectious Diseases 119:126
    [Google Scholar]
  21. Jones A. S., Walker R. T. 1963; Isolation and analysis of the deoxyribonucleic acid of Mycoplasma mycoides var.capri . Nature; London: 198588
    [Google Scholar]
  22. Jones A. S., Tittenson J. R., Walker R. T. 1965; The chemical composition of the nucleic acids and other macromolecular constituents of Mycoplasma mycoides var.capri . Journal of General Microbiology 40:405
    [Google Scholar]
  23. Kelton W.H, Mandel M. 1969; Deoxyribonucleic acid base compositions of Mycoplasma strains of avian origin. Journal of General Microbiology 56:131
    [Google Scholar]
  24. Kenny G. E. 1969; Serological comparison of ten glycolytic Mycoplasma species. Journal of Bacteriology 98:1044
    [Google Scholar]
  25. Leach R. H. 1967; Comparative studies of Mycoplasma of bovine origin. Annals of the New York Academy of Sciences 143:305
    [Google Scholar]
  26. Lemcke R., Forshaw K. A., Fallon R. J. 1969; The serological identity of Sabin’s murine type C Mycoplasma and Mycoplasma pulmonis . Journal of General Microbiology 58:95
    [Google Scholar]
  27. Lemcke R., Marmion B. P., Plackett P. 1967; Immunochemical analysis of Mycoplasma pneumoniae . Annals of the New York Academy of Sciences 143:691
    [Google Scholar]
  28. Mandel M. 1969; New approaches to bacterial taxonomy: Perspective and prospects. Annual Review of Microbiology 23:239
    [Google Scholar]
  29. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from micro-organisms . Journal of Molecular Biology 3:208
    [Google Scholar]
  30. Marmur J., Doty P. 1961; Thermal renaturation of deoxyribonucleic acids. Journal of Molecular Biology 3:585
    [Google Scholar]
  31. Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. Journal of Molecular Biology 5:109
    [Google Scholar]
  32. McGee Z. A., Rogul M., Wittler R. G. 1967; Molecular genetic studies of relationships among Mycoplasma, L-forms and bacteria. Annals of the New York Academy of Sciences 143:21
    [Google Scholar]
  33. McGee Z. A., Rogul M., Falkow S., Wittler R. G. 1965; The relationship of Mycoplasma pneumoniae (Eaton agent) to Streptococcus MG : application of genetic tests to determine relatedness of L-forms and PPLO to bacteria. Proceedings of the National Academy of Sciences of the United States of America 54:457
    [Google Scholar]
  34. Morowitz H. J., Bode H. R., Kirk R. G. 1967; The nucleic acids of Mycoplasma. Annals of New York Academy of Sciences 143:110
    [Google Scholar]
  35. Morowitz H. J., Tourtellotte M. E., Guild W. R., Castro E., Woese C., Cleverdon R. C. 1962; The chemical composition and submicroscopic morphology of Mycoplasma gallisepticum, Avian 5969. Journal of Molecular Biology 4:93
    [Google Scholar]
  36. Neimark H. 1967; Heterogeneity among the Mycoplasma and relationships to bacteria. Annals of the New York Academy of Sciences 143:31
    [Google Scholar]
  37. Neimark H. 1969; Nucleic acid characterization of recently isolated Mycoplasma. Bacteriological Proceedings p. 32
    [Google Scholar]
  38. Neimark H. C., Pene J. J. 1965; Characterization of pleuropneumonia-like organisms by deoxyribonucleic acid composition. Proceedings of the Society for Experimental Biology and Medicine 118:517
    [Google Scholar]
  39. Neimark H., Pickett M. J. 1960; Products of glucose metabolism by pleuropneumonia-like organisms. Annals of the New York Academy of Sciences 79:531
    [Google Scholar]
  40. Olsen N. O., Yamamoto R., Ortmayer H. 1965; Antigenic relationship between Mycoplasma synoviae and Mycoplasma gallisepticum . American Journal of Veterinary Research 26:195
    [Google Scholar]
  41. Peterson A. M., Pollock M. E. 1969; Deoxyribonucleic acid homology and relative genome size in Mycoplasma. Journal of Bacteriogy 99:639
    [Google Scholar]
  42. Pollock M. E., Bonner S. V. 1969; Comparison of saprophytic Mycoplasma by DNA-DNA hybridization. Bacteriological Proceedings p. 32
    [Google Scholar]
  43. Razin S. 1968; Mycoplasma taxonomy studies by electrophoresis OF cell proteins. Journal of Bacteriology 96:687
    [Google Scholar]
  44. Reich P. R., Somerson N. L., Hybner C. J., Chanock R. M., Weisman S. M. 1966; Genetic differentiation by nucleic acid homology. I. Relationships among Mycoplasma species of man. Journal of Bacteriology 92:302
    [Google Scholar]
  45. Rogul M., Mcgee Z. A., Wittler R. G., Falkow S. 1965; Nucleic acid homologies of selected bacteria, L-forms and Mycoplasma species. Journal of Bacteriology 90:1200
    [Google Scholar]
  46. Ryan J. L., Morowitz H. J. 1969; Partial purification of native rRNA and tRNA cistrons from Mycoplasma sp. (kid). Proceedings of the National Academy of Sciences of the United States of America 63:1282
    [Google Scholar]
  47. Saito H., Miura K. 1963; Preparation of transforming deoxyribonucleic acid by phenol treatment. Biochimica et Biophysica Acta 72:619
    [Google Scholar]
  48. Schildkraut C. L., Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its buoyant density in CsCl. Journal of Molecular Biology 4:430
    [Google Scholar]
  49. Shepard M. C. 1967; Cultivation and properties of T-strains of Mycoplasma associated with nongonococcal urethritis. Annals of the New York Academy of Sciences 143:505
    [Google Scholar]
  50. Somerson N. L., Reich P. R., Chanock R. M., Weissman S. M. 1967; Genetic differentiation by nucleic acid homology. III. Relationships among Mycoplasma, L-forms and bacteria. Annals of the New York Academy of Sciences 14:9
    [Google Scholar]
  51. Somerson N. L., Reich P. R., Walls B. E., Chanock R. M., Weissman S. M. 1966; Genetic differentiation by nucleic acid homology. II. Genetic variations within two Mycoplasma species. Journal of Bacteriology 92:311
    [Google Scholar]
  52. Sueoka N. 1961; Variation and heterogeneity of base composition of deoxyribonucleic acids: a compilation of old and new data. Journal of Molecular Biology 3:31
    [Google Scholar]
  53. Taylor-Robinson D., Dinter Z. 1968; Unexpected serotypes of mycoplasmas isolated from pigs. Journal of General Microbiology 53:221
    [Google Scholar]
  54. Taylor-Robinson D., Williams M. H., Haig D. A. 1968; The isolation and comparative biological and physical characteristics of T-mycoplasmas of cattle. Journal of General Microbiology 54:33
    [Google Scholar]
  55. Tully J. G., Razin S. 1968; Physiological and serological comparisons among strains of Mycoplasma granularum and Mycoplasma laidlawii . Journal of Bacteriology 95:1504
    [Google Scholar]
  56. Tully J. G., Razin S. 1969; Characteristics of a new sterol-nonrequiring Mycoplasma. Journal of Bacteriology 98:970
    [Google Scholar]
  57. Walker R. T. 1967; DNA homology of two Mycoplasma species. Nature; London: 216711
    [Google Scholar]
  58. Williams C. O., Wittler R. G., Burris C. 1969; Deoxyribonucleic acid base compositions of selected mycoplasmas and L-phase variants. Journal of Bacteriology 99:341
    [Google Scholar]
/content/journal/micro/10.1099/00221287-63-2-249
Loading
/content/journal/micro/10.1099/00221287-63-2-249
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error