1887

Abstract

SUMMARY: -Glucotransferase was demonstrated in mycelial extracts and culture filtrates of grown on 2 % cellobiose as the sole source of carbon. Analysis of the mycelium indicated relatively large amounts of cellobiose and gentiobiose, as well as small amounts of a series of three oligosaccharides (DP3,4, 5) and glucose; the same compounds were detected in the culture filtrate but at much lower concentrations (except for cellobiose) and glucose was barely detectable. Cellobiose phosphorylase was also present in mycelial extracts, and it is suggested that the production of glucose-1-phosphate from the phosphorylytic cleavage of cellobiose and its rapid utilization as a respiratory substrate explains why the fungus grows so much faster on cellobiose than on glucose. The level of these two enzyme systems is considered to be an important factor in controlling the rate of growth. The significance of the formation of oligosaccharides by the transferase system is in relation to cellulose degradation.

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/content/journal/micro/10.1099/00221287-63-2-175
1970-10-01
2021-10-20
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References

  1. Bacon J.S.D., Edelman J. E. 1951; The carbohydrates of the Jerusalem artichoke and other compositae. Biochemical Journal 48:114–126
    [Google Scholar]
  2. Buston H. W., Khan A. H. 1956; The production of β-linked glucose saccharides from cellobiose by Chaetomium globosum . Biochimica et Biophysica Acta 19:564–565
    [Google Scholar]
  3. Caroselli N. E. 1954; Polysaccharides produced in vitro by Verticillium albo-atrum . Dissertation Abstracts 14:2186–2187
    [Google Scholar]
  4. Chesters C.G.C., Bull A. T. 1964; Transglucosylase activity of fungal laminarinases. Nature; London: 202454–465
    [Google Scholar]
  5. Fiske C. H., Subbarow Y. 1925; The colorimetric determination of phosphorus. Journal of Biological Chemistry 66:375–400
    [Google Scholar]
  6. Green R. J. 1954; A preliminary investigation of toxins produced in vitro by Verticillium albo- atrum . Phytopathology 44:433–437
    [Google Scholar]
  7. Gupta D. P., Eieale J. B. 1970; Induction of cellulase (Cx) in Verticillium albo-atrum . Journal of General Microbiology 63:163–173
    [Google Scholar]
  8. Hanes C. S., Isherwood F. A. 1949; Separation of the phosphoric esters on the filter paper chromatogram. Nature; London: 1641107–1112
    [Google Scholar]
  9. Hulcher F. J., King K. W. 1958; Metabolic basis for disaccharides preference in a Cellvibrio. Journal of Bacteriology 76:571–577
    [Google Scholar]
  10. Le Tourneau D. 1961; Oligosaccharides production from sucrose by Verticillium albo-atrum . Plant Physiology 36:102–104
    [Google Scholar]
  11. Mandels M., Reese E. T. 1960; Induction of cellulase in fungi by cellobiose. Journal of Bacteriology 79:816–826
    [Google Scholar]
  12. Sato M., Takahashi H. 1967; Fermentation of C14-labelled cellobiose by Cellulomonas fimi . Agricultural and Biological Chemistry, Tokio 31:70–74
    [Google Scholar]
  13. Swisher E. J., Storvick W. O., King K. W. 1964; Metabolic nonequivalence of the two glucose metabolites of cellobiose in Cellvibrio gilvus . Journal of Bacteriology 88:817–820
    [Google Scholar]
  14. Whitney P., Chapman J. M., Heale J. B. 1969; Carboxymethycellulase production by Verticillium albo-atrum . Journal of General Microbiology 56:215–225
    [Google Scholar]
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