Summary: Of a large number of sugars and polysaccharides, only cellulose substrates or cellobiose induced Cx (B-i,4-glucan glucano hydrolase; Enzyme Commission no. 3.2.1.4) in Verticillium albo-atrum. Many of the sugars tested inhibited the induction of Cx in the presence of carboxymethyl-cellulose (CM cellulose); this is explained on the basis of a balance between growth and enzyme production such that a low growth rate is usually coupled with high Cx levels and vice versa. One per cent glucose completely repressed Cx induction in 0·1 % cellobiose medium. Cellobiose caused slight inactivation of Cx by end-product inhibition. If the mycelium has no physical contact with insoluble cellulose in liquid cultures, virtually no growth occurs and Cx values are insignificant. These results are discussed in relation to the postulated C1 enzyme involved in the initial stages of cellulose degradation.
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