1887

Abstract

Summary: The effects of varying the concentrations of the nitrogen sources in glycerol + asparagine, glycerol + asparagine + casein hydrolysate and glycerol + ammonium sulphate media on the composition and growth rate of grown in shaken culture for various periods were investigated. The bacteria were fractionated and the soluble, alkali-extractable, hot acid-soluble and residual fractions analysed for carbohydrate, RNA, DNA, free lipid, soluble amino compounds and soluble phosphates.

The results indicate that several forms of growth restriction operated in these experiments: a progressive reduction in growth rate even in conditions when nutrients were not limiting, due to clumping of cells; a reduction in growth rate following any reduction in the amino acid content of the medium; a marked reduction in growth rate when the amino acid content of the medium fell below a critical level, or was replaced by ammonium ions. When the growth rate was reduced because of nutrient limitation, the carbohydrate content of the bacteria increased, but the pattern of accumulation varied with the experimental conditions. The RNA: protein ratio of the cells was little affected by growth rate.

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1970-09-01
2022-05-21
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References

  1. Antoine A. D., Tepper B. S. 1969a; Environmental control of glycogen and lipid content of Mycobacterium phlei . Journal of General Microbiology 55:217–226
    [Google Scholar]
  2. Antoine A. D., Tepper B. S. 1969b; Environmental control of glycogen and lipid content of Mycobacterium tuberculosis . Journal of Bacteriology 100:538–541
    [Google Scholar]
  3. Bowles J. A., Segal W. 1965; Kinetics of utilization of organic compounds in the growth of Mycobacterium tuberculosis . Journal of Bacteriology 90:157–163
    [Google Scholar]
  4. Dubois M., Gilles K., Hamilton J. K., Rebers P. A., Smith F. 1956; Colorimetric method for determination of sugars and related substances. Analytical Chemistry 28:350–356
    [Google Scholar]
  5. Freter R., Ozawa A. 1963; Explanation for limitation of populations of Escherichia coli in broth cultures. Journal of Bacteriology 86:904–910
    [Google Scholar]
  6. Maaløe O., Kjeldgaard N. O. Control of Macromolecular Synthesis New York: W. A.Benjamin;
    [Google Scholar]
  7. Magasanik B., Magasanik A. K., Neidhardt F. C. 1959 In Regulation of Cell Metabolism pp 334–349 Wolstenholme G. E. W., O’Connor C. M. Edited by London: J. and A. Chur-chill. ;
    [Google Scholar]
  8. Maruyama Y., Ono T., Sato T. 1963; Synchronous growth of Mycobacterium smegmatis as induced by cellular crowding. Journal of General and Applied Microbiology 9:41–47
    [Google Scholar]
  9. Moore S., Stein W. H. 1954; A modified ninhydrin reagent for the photometric determination of amino acids and related compounds. Journal of Biological Chemistry 211:907–913
    [Google Scholar]
  10. Neidhardt F. C. 1963; Effects of environment on the composition of bacterial cells. Annual Review of Microbiology 17:61–86
    [Google Scholar]
  11. Soltys M. A. 1952 Tubercle Bacillus and Laboratory Methods in Tuberculosis p. 129 Edinburgh: E. and S.Livingstone;
    [Google Scholar]
  12. Tepper B. S. 1965; Modification of cellular constituents during growth of Mycobacterium phlei . American Review of Respiratory Diseases 92:75–82
    [Google Scholar]
  13. Tsumita T., Chargaff E. 1958; Studies on nucleoproteins. VI. The deoxyribonucleoprotein and the deoxyribonucleic acid of bovine tubercle bacilli (BCG). Biochimica et Biophysica Acta 29:568–578
    [Google Scholar]
  14. Winder F. G., Brennan P. J., Mcdonnell I. 1967; Effects of isoniazid on the composition of mycobacteria, with particular reference to carbohydrates and related substances. Biochemical Journal 104:385–393
    [Google Scholar]
  15. Winder F. G., Denneny J. M. 1956; Phosphorus metabolism of mycobacteria: determination of phosphorus compounds in some mycobacteria. Journal of General Microbiology 15:1–18
    [Google Scholar]
  16. Winder F. G., O’Hara C. 1962; The effects of iron deficiency and of zinc deficiency on the composition of Mycobacterium smegmatis . Biochemical Journal 82:98–108
    [Google Scholar]
  17. Winder F. G., Rooney S. A. 1970; The effects of isoniazid on the carbohydrates of Mycobacterium tuberculosis BCG. Biochemical Journal 117:355–368
    [Google Scholar]
  18. Youmans A. S., Youmans G. P. 1968; Ribonucleic acid, deoxyribonucleic acid, and protein content of cells of different ages of Mycobacterium tuberculosis and the relationship to immunogenicity. Journal of Bacteriology 95:272–279
    [Google Scholar]
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