1887

Abstract

SUMMARY

The homoserine--transsuccinylase activity of three kinds of methionine- excreting mutants of was examined. In a mutant the enzyme was resistant to inhibition by methionine or its analogue α-methyl-methionine. Feedback inhibition in a and a strain was normal. was dominant to only when coupled in the position with the wild-type allele of the closely-linked (homoserine--transsuccinylase) gene, and a deletion analysis of nine mutations showed that they were all located within the gene. Thus both the regulatory and catalytic sites of homoserine--transsuccinylase are specified by a single polypeptide species. An estimate was made of the length of the gene, based on recombination data.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-63-1-111
1970-09-01
2021-08-03
Loading full text...

Full text loading...

/deliver/fulltext/micro/63/1/mic-63-1-111.html?itemId=/content/journal/micro/10.1099/00221287-63-1-111&mimeType=html&fmt=ahah

References

  1. Ames G. F., Roth J. R. 1968; Histidine and aromatic permeases of Salmonella typhimurium . Journal of Bacteriology 96:1742–1749
    [Google Scholar]
  2. Ayling P. D., Chater K. F. 1968; The sequence of four structural and two regulatory methionine genes in the Salmonella typhimurium linkage map. Genetical Research 12:341–354
    [Google Scholar]
  3. Balbinder E. 1964; Intergenic complementation between A and B components of bacterial tryptophan synthetases. Biochemical and Biophysical Research Communications 17:770–774
    [Google Scholar]
  4. Chater K. F. 1969 Linkage and Dominance Studies of Genes Controlling Methionine Synthesis in Salmonella typhimurium. Ph.D.Thesis Birmingham University:
    [Google Scholar]
  5. Chater K. F. 1970a; Dominance of the wild-type alleles of methionine regulatory genes in Salmonella typhimurium . Journal of General Microbiology 63:95–109
    [Google Scholar]
  6. Chater K. F. 1970b; A genet ical study of feedback inhibition of methionine synthesis in Salmonella typhimurium . Heredity 25:154
    [Google Scholar]
  7. Childs J. D., Smith D. A. 1969; New methionine structural gene in Salmonella typhimurium . Journal of Bacteriology 100:377–382
    [Google Scholar]
  8. Demerec M., Adelberg E. A., Clark A. J., Hartman P. E. 1966; A proposal for a uniform nomenclature in bacterial genetics. Genetics, Princeton 54:61–76
    [Google Scholar]
  9. Fink G. R., Roth J. R. 1968; Histidine regulatory mutants in Salmonella typhimurium. VI. Dominance studies. Journal of Molecular Biology 33:547–557
    [Google Scholar]
  10. Gerhart J. C., Schachman H. K. 1965; Distinct subunits for the regulation and catalytic activity of aspartate transcarbamylase. Biochemistry 4:1054–1062
    [Google Scholar]
  11. Hershey A. D., Chase M. 1952; Independent function of viral protein and nucleic acid in the growth of bacteriophage. Journal of General Physiology 36:39–51
    [Google Scholar]
  12. Ito J. 1969; Hybrid anthranilate synthetase molecules from Enterobacterial sources. Nature; London: 22357–59
    [Google Scholar]
  13. Lawrence D. A. 1967 Methionine Analogue-resistant Mutants of Salmonella typhimurium. Ph.D.Thesis Birmingham University:
    [Google Scholar]
  14. Lawrence D. A., Smith D. A., Rowbury R. J. 1968; Regulation of methionine synthesis in Salmonella typhimurium: mutants resistant to inhibition by analogues of methionine. Genetics, Princeton 58:473–492
    [Google Scholar]
  15. Lee L. W., Ravel J. M., Shive W. 1966; Multimetabolite control of a biosynthetic pathway by sequential metabolites. Journal of Biological Chemistry 241:5479–5480
    [Google Scholar]
  16. Rowbury R. J. 1964; Synthesis of cystathionine and its control in Salmonella typhimurium . Nature; London: 203977–978
    [Google Scholar]
  17. Rowbury R. J. 1968; The inhibitory action of a-methylmethionine on Escherichia coli . Journal of General Microbiology 52:223–230
    [Google Scholar]
  18. Rowbury R. J., Woods D. D. 1964; O-SuccinyIhomoserine as an intermediate in the synthesis of cystathionine by Escherichia coli . Journal of General Microbiology 36:341–358
    [Google Scholar]
  19. Sanderson K. E. 1967; Revised linkage map of Salmonella typhimurium . Bacteriological Reviews 31:354–372
    [Google Scholar]
  20. Schlesinger S. 1967; Inhibition of growth of Escherichia coli and of homoserine-O-transsuccinylase by α-methylmethionine. Journal of Bacteriology 94:327–332
    [Google Scholar]
  21. Sheppard D. E. 1964; Mutants of Salmonella typhimurium resistant to feedback inhibition by l-histidine. Genetics, Princeton 50:611–623
    [Google Scholar]
  22. Smith D. A. 1961; Some aspects of the genetics of methionineless mutants of Salmonella typhimurium . Journal of General Microbiology 24:335–353
    [Google Scholar]
  23. Smith D. A., Childs J. D. 1966; Methionine genes and enzymes of Salmonella typhimurium . Heredity 21:265–286
    [Google Scholar]
  24. Smith R. C. 1968; Growth and nucleic acid synthesis of Salmonella typhimurium inhibited by α-methylmethionine. Canadian Journal of Microbiology 14:331–335
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-63-1-111
Loading
/content/journal/micro/10.1099/00221287-63-1-111
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error