
Full text loading...
SUMMARY
The homoserine-O-transsuccinylase activity of three kinds of methionine- excreting mutants of Salmonella typhimurium was examined. In a metI mutant the enzyme was resistant to inhibition by methionine or its analogue α-methyl-methionine. Feedback inhibition in a metJ and a metK strain was normal. metI was dominant to metI+ only when coupled in the cis position with the wild-type allele of the closely-linked metA (homoserine-O-transsuccinylase) gene, and a deletion analysis of nine metI mutations showed that they were all located within the metA gene. Thus both the regulatory and catalytic sites of homoserine-O-transsuccinylase are specified by a single polypeptide species. An estimate was made of the length of the metA gene, based on recombination data.
Article metrics loading...
Full text loading...
References
Data & Media loading...