1887

Abstract

SUMMARY: A B locus, determining E2 refractivity at low temperature, has been mapped at minute 89 on the 12 chromosome and is 94 % linked to the loci. A second locus, A, which controls expression of B in some way, was found to be 70 % co-transducible with and to the right of Both B and C, another locus which determines E2 refractivity, are 21 % co-transducible with B and the C locus therefore also probably lies close to Mutations of the C locus appear to be pleiotropic and the additional characteristics of these mutants, u.v. sensitivity, detergent sensitivity, recombination deficiency, filament formation, and abortive λ growth, are all fully co-transducible with E 2-refractivity. In addition, u.v.-resistant revertants of CetC mutants simultaneously revert to all wild-type characteristics including E 2 sensitivity. The nature of the recombination deficiency of some CetC mutants has been examined and both episomal and chromosomal gene transfer to the mutants found to be normal. P1 transduction frequencies with CetC mutants are, however, also normal and it is suggested that the mutants may be deficient in some recombination step unique to Hfr × F crosses.

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/content/journal/micro/10.1099/00221287-62-3-383
1970-08-01
2024-04-25
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References

  1. Anderson H. 1946; Growth requirements of virus resistant mutants of a bacterial strain. Proceedings of the National Academy of Sciences of the United States of America 32:120–128
    [Google Scholar]
  2. Arber W. 1968; Host controlled restriction and modification of bacteriophage. Symposia of the Society for General Microbiology 18:295–3I4
    [Google Scholar]
  3. Emmerson P. T. 1968; Recombination deficient mutants of Escherichia coli k12 that map between thyA and argA. Genetics, Princeton 60:19–30
    [Google Scholar]
  4. Emmerson P. T., Howard-Flanders P. 1967; Co-transduction with thy of a gene required for genetic recombination in Escherichia coli . Journal of Bacteriology 93:1729–1731
    [Google Scholar]
  5. Ganesan A. K., Smith K. C. 1968; Dark recovery processes in Escherichia coli irradiated with ultraviolet light. 1. Effect of rec mutations on liquid holding recovery. Journal of Bacteriology 96:365–372
    [Google Scholar]
  6. Glover S. W. 1962; Valine resistant mutants of Escherichia coli k12. Genetical Research 3:448
    [Google Scholar]
  7. Glover S. W., Colson C. 1969; Genetics of host-controlled restriction and modification in Escherichia coli . Genetical Research 13:227–240
    [Google Scholar]
  8. Hill C., Holland I. B. 1967; Genetic basis of colicin E susceptibility in Escherichia coli I. Isolation and properties of refractory mutants and the preliminary mapping of their mutations. Journal of Bacteriology 94:677–686
    [Google Scholar]
  9. Holland I. B. 1967; The properties of u.v.-sensitive mutants of Escherichia coli k 12 which are also refractory to colicin E2. Molecular and General Genetics 100:242–251
    [Google Scholar]
  10. Holland I. B. 1968; Properties of Escherichia coli K 12 mutants which show conditional refractivity to colicin E2. Journal of Molecular Biology 31:267–275
    [Google Scholar]
  11. Holland I. B., Threlfall E. J. 1969; Identification of closely linked loci controlling ultraviolet sensitivity and refractivity to colicin E2 in Escherichia coli . Journal of Bacteriology 97:91–96
    [Google Scholar]
  12. Holland I. B., Threlfall E. J., Holland E. M., Darby V., Samson A. C. R. 1970; Mutants of Escherichia coli with altered surface properties which are also refractory to colicin E 2, sensitive to ultraviolet light and which can also show recombination deficiency, abortive growth of bacteriophage A and filament formation. Journal of General Microbiology 62:371–382
    [Google Scholar]
  13. Low B. 1968; Formation of merodiploids in matings with a class of Rec recipient strains of Escherichia coli k12. Proceedings of the National Academy of Sciences of the United States of America 60:160–167
    [Google Scholar]
  14. Luria S. E., Hertman I. 1967; Transduction studies on the role of a rec+ gene in the ultraviolet induction of prophage lambda. Journal of Molecular Biology 23:117–133
    [Google Scholar]
  15. Meselson M., Yuan R. 1968; DNA restriction enzyme from Escherichia coli . Nature; London: 2171110–1114
    [Google Scholar]
  16. Pardee A., Jacob F., Monod J. 1959; The genetic control and cytoplasmic expression of ‘inducibility’ in the synthesis of β-galactosidase. Journal of Molecular Biology 1:165–178
    [Google Scholar]
  17. Ryter A. 1968; Association of the nucleus and the membrane of bacteria: a morphological study. Bacteriological Review 32:39–54
    [Google Scholar]
  18. Sueoka N., Quinn W. G. 1968; Membrane attachment of the chromosome replication origin in Bacillus subtilis . Cold Spring Harbor Symposia on Quantitative Biology 33:695–705
    [Google Scholar]
  19. Taylor A. L., Trotter C. D. 1967; Revised linkage map of Escherichia coli . Bacteriological Reviews 31:332–353
    [Google Scholar]
  20. Willetts N., Clark A. J. 1969; Characteristics of some multiple recombination deficient strains of Escherichia coli . Journal of Bacteriology 100:231–239
    [Google Scholar]
  21. Wu T. T. 1966; A model for three-point analysis of random general transduction. Genetics, Princeton 54:405–410
    [Google Scholar]
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