SUMMARY: A method for the isolation of mycococci directly from cultures of is described. The method consists of the cultivation of in Middlebrook's 7H9 liquid medium without the addition of any enrichments. The medium was distributed in screw-capped bottles, plugged for 3 weeks with cotton-wool only and then for another 4 weeks with the caps screwed on top of the cotton-wool plugs. The mycococci were then isolated on subcultures on nutrient agar. A streptomycin-resistant variant of yielded a mycococcus that was streptomycin-resistant. Frequent sub-cultivation of the parent strain probably prevented the development of mycococci. Evidence is submitted that the mycococci were not contaminants.


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