1887

Abstract

SUMMARY

A method for the isolation of mycococci directly from cultures of is described. The method consists of the cultivation of in Middlebrook’s 7H9 liquid medium without the addition of any enrichments. The medium was distributed in screw-capped bottles, plugged for 3 weeks with cotton-wool only and then for another 4 weeks with the caps screwed on top of the cotton-wool plugs. The mycococci were then isolated on subcultures on nutrient agar. A streptomycin-resistant variant of yielded a mycococcus that was streptomycin-resistant. Frequent subcultivation of the parent strain probably prevented the development of mycococci. Evidence is submitted that the mycococci were not contaminants.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-62-2-251
1970-08-01
2022-01-29
Loading full text...

Full text loading...

/deliver/fulltext/micro/62/2/mic-62-2-251.html?itemId=/content/journal/micro/10.1099/00221287-62-2-251&mimeType=html&fmt=ahah

References

  1. A System of Bacteriology in Relation to Medicine 1931; 9 p. 60 Edited by the Medical Research Council London: H.M. Stationery Office;
  2. Austwick P. K. C. 1968; Polymorphism in fungi. Journal of General Microbiology 50:xv
    [Google Scholar]
  3. Bergey’s Manual of Determinative Bacteriology 1957, 7th edn. pp. 707–771 Breed R. S., Murray E. G., Smith N. R. Edited by Baltimore: Williams and Wilkins;
  4. Brieger E. M., Glauert A. M. 1956; Spore-like structures in the tubercle bacillus. Nature; London: 178544–545
    [Google Scholar]
  5. Cruickshank R. 1965 Medical Microbiology, 11th edn. pp. 649–652 Edinburgh: E. and S. Livingstone;
    [Google Scholar]
  6. Csillag A. 1961; Spore formation and ‘dimorphism’ in the mycobacteria. Journal of General Microbiology 26:97–109
    [Google Scholar]
  7. Csillag A. 1962; Development of form 2 mycobacteria on autoclaved Loewenstein-Jensen medium. Tubercle; London: 43439–443
    [Google Scholar]
  8. Csillag A. 1963a; Cellular morphology of form 2 mycobacteria in slide culture. Journal of General Microbiology 30:21–27
    [Google Scholar]
  9. Csillag A. 1963b; Stabilization of various forms of Mycobacterium tuberculosis . Tubercle; London: 44368–371
    [Google Scholar]
  10. Csillag A. 1964a; Growth of form 2 mycobacterium and various Bacillus species on Loewenstein-Jensen medium. Journal of General Microbiology 34:79–83
    [Google Scholar]
  11. Csillag A. 1964b; The mycococcus form of mycobacteria. Journal of General Microbiology 34:341–352
    [Google Scholar]
  12. Demikhovskii I. E. 1961; The species composition of the bacterial antagonists of the oral cavity. Journal of Microbiology, Epidemiology and Immunobiology 32:1529–1533 In Russian
    [Google Scholar]
  13. Hilson G. R. F. 1965; Taxonomic characteristics of so-called ‘form 2 mycobacteria’. Journal of General Microbiology 39:407–421
    [Google Scholar]
  14. Howard D. H. 1962; The morphogenesis of the parasitic forms of dimorphic fungi. Mycopathologia and Mycologia Applicata 18:127–139
    [Google Scholar]
  15. Juhasz S. E. 1962; Aberrant forms of Mycobacterium phlei produced by streptomycin and their multiplication on streptomycin-free media. Journal of General Microbiology 28:9–13
    [Google Scholar]
  16. Kobayashi G. S., Guiliacci P. L. 1967; Cell wall studies of Histoplasma capsulatum . Sabouraudia 5:180–188
    [Google Scholar]
  17. Krassilnikov N. A. 1934; Die Entwicklungsgeschichte der Bodenmikrobakterien. Zentralblatt für Bakteriologie 90:428–434
    [Google Scholar]
  18. Krassilnikov N. A. 1938 Microbiologia 7: part I p. 335 In Russian
    [Google Scholar]
  19. Krassilnikov N. A. 1959 Diagnostik der Bakterien und Actinomyceten pp. 39–130203–204542–555 Jena: Gustav Fischer;
    [Google Scholar]
  20. Lack C. H., Tanner F. 1953; The significance of pleomorphism in Mycobacterium tuberculosis var. hominis . Journal of General Microbiology 8:18–28
    [Google Scholar]
  21. Pease P. 1970; An acid-fast bacillary phase in Streptococcus mg and certain other Gram-positive cocci: identification with Mycococcus (Krassilnikov). Journal of Medical Microbiology 3:186–189
    [Google Scholar]
  22. Stewart-Tull D. E. S. 1965; Occurrence of dimorphic forms of Mycobacterium phlei . Nature; London: 208603–605
    [Google Scholar]
  23. Tepper B. S. 1965; Modification of cellular constituents during growth of Mycobacterium phlei . American Review of Respiratory Diseases 92:75–82
    [Google Scholar]
  24. Topley and Wilson’s Principles of Bacteriology and Immunity 1964, 5th edn. pp. 415–5361019 London: Edward Arnold;
  25. Waksman S. A. 1959 The Actinomycetes p. 71 London: Baillière, Tindall and Cox;
    [Google Scholar]
  26. Waksman S. A. 1961 The Actinomycetes 2 p. 331 London: Baillière, Tindall and Cox;
    [Google Scholar]
  27. Xalabarder C. 1954 El origen del Bacilo de Koch pp. 92–94 Barcelona: Instituto Antituberculoso ‘Francisco Moragas’;
    [Google Scholar]
  28. Xalabarder C. 1958; Electron microscopy of tubercle bacilli. Excerpta Medica 11:467
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-62-2-251
Loading
/content/journal/micro/10.1099/00221287-62-2-251
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error