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SUMMARY: The appearance of the walls of apical and sub-apical regions of hyphae of predominantly 5 day cultures of Neurospora crassa, Schizophyllum commune and Phytophthora parasitica as seen with the electron microscope, employing shadowed or sectioned material, is illustrated and described in detail. The appearance of untreated or control material in buffer is compared with that exposed to various single and sequential treatments with enzymes, including laminarinase, Pronase, cellulase and chitinase, as well as various chemical treatments.
From these observations is inferred the co-axial distribution of polymers such as β1,3-, β1,6-glucans, glycoproteins, proteins, chitin and cellulose in the walls of each species. The distributions have both similarities and differences between the species. The significance of all these features for the growth, mechanical rigidity and integrity of a hypha is briefly discussed.
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