SUMMARY: An unbalanced growth situation, resulting in large, plasmolysed sphero-plasts (megalomorphs) produced by the addition of nickel to the growth medium of a marine bacterium, Arthrobacter marinus sp.nov., is described.The effect of increasing nickel concentrations was gradual. Under the cultural conditions used, a slight effect was noted on size in 1 × 10−4m-NiCl2, but in 4 × 10−4m-NiCl2 the cells formed megalomorphs attaining a size of 10 to 15 μm. Cell division did not occur in 5 × 10−4m-nickel. The lag phase of the culture increased from about 3 h. without added nickel to more than 70 h. at 4 × 10−4m-nickel. Concurrently, the maximum population decreased as the nickel concentration was increased. The classification of the marine bacterium is described and the organism is designated as A. marinus sp.nov.
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