1887

Abstract

SUMMARY: In the hypha of the succinic dehydrogenase activity is localized in the mesosomes, plasma membrane and mitochondria. Short permanganate fixation after incubation enhances the positive cyto-chemical reaction. The higher activity is localized in mesosomes and it is observed as high electron-dense zones and concentric ring-like patterns.The membrane shows a spotty reaction and the mitochondrion shows a positive zone in the intracristae space.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-62-1-43
1970-07-01
2022-05-21
Loading full text...

Full text loading...

/deliver/fulltext/micro/62/1/mic-62-1-43.html?itemId=/content/journal/micro/10.1099/00221287-62-1-43&mimeType=html&fmt=ahah

References

  1. Bradbury S., Mek G. 1690; A study of potassium permanganate fixation for electron microscopy. Quarterly Journal of Microscopical Science 101:241
    [Google Scholar]
  2. Carbonell L. M., Kanetsuna F. 1966; Nature of the intrinsic factors which reduced tetrazolium salt in the histochemical demonstration of dehydrogenases in the fungus Paracoccidioides brasiliensis . Annales d’Histochimie 11:375
    [Google Scholar]
  3. Carbonell L. M., Rodriguez J. 1968; Mycelial phase of Paracoccidioides brasiliensis and Blastomyces dermatitidis: an electron microscope study. Journal of Bacteriology 96:533
    [Google Scholar]
  4. Cesari I. M., Rieber M., Imaeda T. 1969; Localization and properties of enzymes involved with electron transport activity in mycobacteria. Journal of Bacteriology 98:767
    [Google Scholar]
  5. Edwards G. A., Edwards M. R. 1960; The intracellular membrane of Blastomyces dermatitidis . American Journal of Botany 47:622
    [Google Scholar]
  6. Ferrandez B., Chaix P., Ryter A. 1966; Localization des cytochromes de Bacillus subtilis dans les structures mesosomique. Compte Rendu Hebdomadaire des Séances de l’Academie des Sciences, Paris 63:1632
    [Google Scholar]
  7. Freeman J. M., Spurlock B. O. 1962; A new epoxy embedment for electron microscopy. Journal of Cell Biology 13:437
    [Google Scholar]
  8. Hopwood D. 1969; Fixatives and fixation: a review. Histochemistry 1:323
    [Google Scholar]
  9. Kellenberg E., Ryter A., Séchaud J. 1958; Electron microscope study of DNA-containing plasms. II. Vegetative and mature phage DNA as compared with normal bacterial nucleoids in different physiological states. Journal of Biophysical and Biochemical Cytology 4:671
    [Google Scholar]
  10. Mollenhauer H. H. 1959; Permanganate fixation of plant cells. Journal of Biophysical and Biochemical Cytology 4:671
    [Google Scholar]
  11. Moore R. T., McAlear J. 1961; Fine structure of mycota 5. Lomasomes, previously uncharacterized hyphal structures. Micologia 53:194
    [Google Scholar]
  12. Ogawa K., Barnett R. J. 1964; Electron histochemical examination of oxidative enzymes and mitochondria. Nature; London: 203724
    [Google Scholar]
  13. Reaveley D. A., Rogers H. S. 1969; Some enzymatic activities and chemical properties of the mesosomes and cytoplasmic membranes of Bacillus licheniformis 6346. Biochemical Journal 113:67
    [Google Scholar]
  14. Reiss J. 1967a; Cytochemical demonstration of glutathione reductase in Saccharomyces cerevisiae . Journal of Histochemistry and Cytochemistry 15:273
    [Google Scholar]
  15. Reiss J. 1967b; Der cytochemische Nachweis von Cytochromoxydase und Peroxydasen bei Pilzen. Histochemie 9:281
    [Google Scholar]
  16. Reiss J. 1968; Grundsatzliches zum cytochemischen Nachweis von Dehydrogenasen bei Pilzen. Zentralblatt für Bakteriologie Parasitenkunde, Infektionskrankheiten und Hygiene 207:231
    [Google Scholar]
  17. Sedar A. W., Burde R. M. 1965; The demonstration of the succinic dehydrogenase system in Bacillus subtilis using tetranitro-blue tetrazolium combined with techniques of electron microscopy. Journal of Cell Biology 27:53
    [Google Scholar]
  18. Sedar A. W., Rosa C., Tsou K. 1962; Tetranitro-blue tetrazolium and the electron histo-chemistry of succinic dehydrogenase. Journal of Histochemistry and Cytochemistry 10:506
    [Google Scholar]
  19. Seligman A. M., Ueno H., Morizono Y., Wasserkrug H., Katzoff L., Hanker J. 1967; Electron microscopic demonstration of dehydrogenase activity with a new osmiophilic ditetrazolium salt (TC-NBT). Journal of Histochemistry and Cytochemistry 15:1
    [Google Scholar]
  20. Vanderwinkel E., Murray R. G. E. 1962; Organelles intracytoplasmiques bactériens et site d’activité oxydo-reductrice. Journal of Ultrastructure Research 7:195
    [Google Scholar]
  21. van Iterson W., Leene W. 1964; A cytochemical localization of reductive sites in a Gram-positive bacterium. Tellurite reduction in Bacillus subtilis . Journal of Cell Biology 20:361
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-62-1-43
Loading
/content/journal/micro/10.1099/00221287-62-1-43
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error