1887

Abstract

SUMMARY

Three methods of detecting respiratory variation in have been investigated. They involved the addition of dyes (pontamine sky blue and eosin yellowish) tellurite or tetrazolium to a complete medium containing ethionine. The latter prevented conidiation and enabled the colours of the colonies to be seen clearly. These methods were used to distinguish nuclear and cytoplasmic cytochrome mutants ( and ) from wild-type colonies on the basis of colour. Tetrazolium violet (2-5-diphenyl-3-(-napthyl)-tetrazolium chloride) incorporated into modified Nagai medium with ethionine is recommended for distinguishing cytoplasmic mutants of Neurospora from wild-type. Certain other nuclear mutants were also distinguishable from wild-type in some of these tests. These were Thirty-two wild-type strains in reciprocal crosses were tested on these media to find whether these tests would distinguish any nuclear or cytoplasmic variation. No new cytoplasmic variants were found. Three new nuclear genes, (mauve on dye media), (ability to reduce tetrazolium) and (suppressor of ) are described and have been mapped. All three loci were linked to the mating type locus on chromosome 1. was found in only certain wild-type strains of mating type (e.g. 74-8-a), and (unable to reduce tetrazolium) was found in all wild-type strains of mating type (e.g. 74-23- ) and certain wild-types of mating type a suppressor of also suppressed and but not suppressed but none of the other cytoplasmic mutants.

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1970-06-01
2021-10-19
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References

  1. Andreasen A. A., Steer T. J. B. 1953; Anaerobic nutrition of Saccharomyces cerevisiae. 1. Ergo-sterol requirement for growth in a defined medium. Journal of Cellular and Comparative Physiology 41:23–36
    [Google Scholar]
  2. Bachmann B. J., Strickland W. N. 1965 Neurospora Bibliography and Index New Haven & London: Yale University Press;
    [Google Scholar]
  3. Barratt R. W. 1962; Origin of important wild-type stocks of N. crassa. . Neurospora Newsletter 224–25
    [Google Scholar]
  4. Barratt R. W. 1968; Further notes on nomenclature: extrachromosomal mutants. Neurospora Newsletter1211
    [Google Scholar]
  5. Bertrand H., McDougall K. J., Pittenger T. H. 1968; Somatic cell variation during uninterrupted growth of Neurospora crassa in continuous growth tubes. Journal of General Microbiology 50:337–350
    [Google Scholar]
  6. Bertrand H., Pittenger T. H. 1964; Cytoplasmic mutants from a Neurospora culture, maintained by continuous hyphal growth. (Abstract). Genetics 50:235
    [Google Scholar]
  7. Bernstein H. 1961; Imidazole compounds accumulated by purine mutants of Neurospora crassa . Journal of General Microbiology 25:41–46
    [Google Scholar]
  8. British Drug Houses Handbook no date Tetrazolium salts.
  9. Brock T. D. 1958; Inhibition of growth of yeasts by 2,3,5-triphenyl tetrazolium chloride. Naturwissenschaften 45:216
    [Google Scholar]
  10. Brody S., Tatum E. L. 1966; The primary biochemical effect of a morphological mutation in Neurospora crassa . Proceedings of the National Academy of sciences of the United States of America 56:1290–1297
    [Google Scholar]
  11. Case M. E., Brockman H. E., Deserres F. J. 1965; Further information on the origin of the Yale and Oak Ridge wild-type strains of Neurospora crassa. . Neurospora Newsletter825–26
    [Google Scholar]
  12. Chang L. T., Tuveson R. W. 1967; Ultraviolet sensitive mutants in Neurospora crassa . Genetics 56:801–810
    [Google Scholar]
  13. Crocken B., Tatum E. L. 1968; The effect of sorbose on metabolism and morphology of Neurospora . Biochimica et Biophysica Acta 156:1–8
    [Google Scholar]
  14. Denny F. E. 1933; Oxygen requirements of Neurospora sitophila for formation of perithecia and growth of mycelium. Contributions. Boyce Thompson Institute for Plant Research 5:95–102
    [Google Scholar]
  15. Diacumakos E. G., Garnjobst L., Tatum E. L. 1965; A cytoplasmic character in Neurospora crassa. The role of nuclei and mitochondria. Journal of Cell Biology 26:427–443
    [Google Scholar]
  16. Ephrussi B. 1953 Nucleo-cytoplasmic Relations in Micro-organisms: Their Bearing on Cell Heredity and Differentiation Oxford: Clarendon Press;
    [Google Scholar]
  17. Fitzgerald P. H. 1963; Genetic and epigenetic factors controlling female sterility in Neurospora crassa . Heredity, London 18:47–62
    [Google Scholar]
  18. Foster J. W., Goldman A. 1948 Chemical Activities of Fungi p. 316 Foster J. W. Edited, by New York: Academic Press;
    [Google Scholar]
  19. Garnjobst L., Wilson J. F., Tatum E. L. 1965; Studies on a cytoplasmic character. Journal of Cell Biology 26:412–425
    [Google Scholar]
  20. Gowdridge B. 1956; Heterokaryons between strains of Neurospora crassa with different cytoplasms. Genetics 41:780–789
    [Google Scholar]
  21. Griffiths A. J. F., Bertrand H., Pittenger T. H. 1968; Cytochrome spectra of cytoplasmic mutants in Neurospora . Neurospora Newsletter1316–17
    [Google Scholar]
  22. Grindle M., Woodward D. O. 1968; Variations among extrachromosomal mutants of N. crassa. . Neurospora Newsletter129–10
    [Google Scholar]
  23. Gross S. R. 1950; Heterokaryons between opposite mating types of Neurospora crassa . Biological Bulletin. Marine Biological Laboratory Woods Hole, Mass 99:331–332
    [Google Scholar]
  24. Horne P., Wilkie D. 1966; Use of magdala red for the detection of auxotrophic mutants of Saccharomyces cerevisiae . Journal of Bacteriology 91:1388
    [Google Scholar]
  25. Houghton J. S. 1967; Respiratory deficient mutants of Aspergillus nidulans . Aspergillus Newsletter8
    [Google Scholar]
  26. Hsu K. S. 1965; Acriflavin resistance controlled by chromosomal genes in Neurospora. Neurospora Newsletter84–6
    [Google Scholar]
  27. Kappy M. S., Metzenberg R. L. 1965; Studies on the basis of ethionine resistance in Neurospora. Biochimica et Biophysica Acta 107:425–433
    [Google Scholar]
  28. Laskowski W. 1954; Induction, par le chlorue de tetrazolium de la mutation ‘petite colonie’ chez la levure. Heredity, London 8:79–88
    [Google Scholar]
  29. Lindegren C. C. 1956; Mutation and other variations in organisms. Comptes rendus des travaux du Laboratoire 26:253–271
    [Google Scholar]
  30. Martin P. G. 1959; Apparent self-fertility in Neurospora crassa . Journal of General Microbiology 20:213
    [Google Scholar]
  31. Mcdougall K. J., Pittenger T. H. 1966; A cytoplasmic variant of Neurospora crassa . Genetics 54:551–565
    [Google Scholar]
  32. Mitchell M. B., Mitchell H. K. 1952; A case of maternal inheritance in Neurospora crassa . Proceedings of the National Academy of sciences of the United States of America 38:442–449
    [Google Scholar]
  33. Mitchell M. B., Mitchell H. K. 1956; A nuclear gene suppressor of a cytoplasmically inherited character in Neurospora crassa . Journal of General Microbiology 14:84–89
    [Google Scholar]
  34. Mitchell M. B., Mitchell H. K., Tissiéres A. 1953; Mendelian and non-Mendelian factors affecting the cytochrome system in Neurospora crassa . Proceedings of the National Academy of sciences of the United States of America 39:606–613
    [Google Scholar]
  35. Nagai S. 1963a; Diagnostic colour differentiatoin plates for hereditary respiration deficiency in yeast. Journal of Bacteriology 86:299–302
    [Google Scholar]
  36. Nagai S. 1963b; Aerobiosis requirement of diagnostic colour differentiation for respiration de-ficiency in yeast. Journal of Bacteriology 86:1135–1136
    [Google Scholar]
  37. Nagai S. 1965a; Variety of diagnostic dye mixtures for respiration deficiency in yeast. Journal of Bacteriology 89:897–898
    [Google Scholar]
  38. Nagai S. 1965b; Differential reduction of tellurite by growing colonies of normal yeast and respiration deficient mutants. Journal of Bacteriology 90:220–222
    [Google Scholar]
  39. Nagai S., Yanagishima N., Nagai H. 1961; Advances in the study of respiration deficient (RD) mutation in yeast and other micro-organisms. Bacteriological Reviews 25:404–426
    [Google Scholar]
  40. Nickerson W. J. 1953; An enzymatic locus participating in cellular division of yeast. Journal of General Physiology 37:483–494
    [Google Scholar]
  41. Ogur M., St John R. Jun. 1957; Tetrazolium overlay technique for population studies of respiration deficiency in yeast. Science; New York: 125928–929
    [Google Scholar]
  42. Ohnishi E., Macleod H., Horowitz N. H. 1962; Mutants of Neurospora deficient in d-amino acid oxidase. Journal of Biological Chemistry 237:138–142
    [Google Scholar]
  43. Pearce A. G. E. 1960 Histochemistry-Theoretical and Applied London: J. &. A Churchill;
    [Google Scholar]
  44. Perkins D. D., Glassey M., Bloom B. A. 1962; New data on markers and rearrangements in Neurospora. Canadian Journal of Genetics and Cytology 4:187–205
    [Google Scholar]
  45. Pittenger T. H. 1956; Synergism of two cytoplasmically inherited mutants in Neurospora crassa . Proceedings of the National Academy of sciences of the United States of America 42:747–752
    [Google Scholar]
  46. Reich E., Luck D. J. L. 1966; Replication and inheritance of mitochondrial DNA. Proceedings of the National Academy of sciences of the United States of America 55:1600–1608
    [Google Scholar]
  47. Sorger G. J. 1963; TPNH-cytochrome c reductase and nitrate reductase in mutant and wild-type Neurospora and Aspergillus. Biochemical and Biophysical Research Communications 12:395–401
    [Google Scholar]
  48. Spence K. D., Parks L. W., Shapiro S. K. 1967; Dominant mutation for ethionine resistance in Saccharomyces cerevisiae . Journal of Bacteriology 94:1531–1537
    [Google Scholar]
  49. Srb A. M. 1963; Extra-chromosomal factors in the genetic differentiation of Neurospora. Symposia of the Society for Experimental Biology 18:175–187
    [Google Scholar]
  50. Stekol J. A. 1963; Biochemical basis for ethionine effects on tissues. Advances in Enzymology 25:369–393
    [Google Scholar]
  51. Strauss B. S. 1958; Cell death and unbalanced growth in Neurospora. Journal of General Microbiology 18:658–669
    [Google Scholar]
  52. Thomas D. Y., Wilkie D. 1968; Recombination of mitochondrial drug-resistance factors in Saccharomyces cerevisiae . Biochemical and Biophysical Research Communications 30:368–372
    [Google Scholar]
  53. Wilson J. F. 1967; Differential effect of 2,3,5-triphenyl tetrazolium chloride on poky and wild-type colonies of Neurospora crassa. (Abstract.). Genetics 56:597
    [Google Scholar]
  54. Woodward D. O., Munkres K. D. 1966; Alterations of a maternally inherited mitochondrial structural protein in respiratory deficient strains of Neurospora. Proceedings of the National Academy of sciences of the United States of America 55:872–880
    [Google Scholar]
  55. Zalokar M. 1953; Reduction of selenite by Neurospora. Archives of Biochemistry and Biophysics 44:330–337
    [Google Scholar]
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