1887

Abstract

Surmmary

Invertase released from (Jorgensen) by either breakage or thiol treatment exhibited identical electrophoretic characteristics. Evidence was obtained against an indirect chemical effect of breakage on the release of invertase from yeasts. Electron microscopy revealed no obvious differences between walls of and other yeasts. A model is proposed in which invertase is not bonded to the wall but is retained by structures requiring intact S—S linkings.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-61-3-327
1970-06-01
2021-08-06
Loading full text...

Full text loading...

/deliver/fulltext/micro/61/3/mic-61-3-327.html?itemId=/content/journal/micro/10.1099/00221287-61-3-327&mimeType=html&fmt=ahah

References

  1. Agar H. D., Douglas H. C. 1955; Study of budding and cell wall structure of yeast. Journal of Bacteriology 70:427–434
    [Google Scholar]
  2. Bacon J. S. D., Milne B. D., Taylor I. F., Webley D. M. 1965; Features of the cell wall structure of yeast revealed by the action of enzymes from a non-fruiting Myxobacterium (Cyto-phagajohnsonii). Biochemical Journal 95:28C
    [Google Scholar]
  3. Burger M., Bacon E. E., Bacon J. S. D. 1958; Liberation of invertase from disintegrated yeast cells. Nature; London: 1821508
    [Google Scholar]
  4. Burger M., Bacon E. E., Bacon J. S. D. 1961; Some observations on the form and location of invertase in the yeast cell wall. Biochemical Journal 78:504–511
    [Google Scholar]
  5. Conti S. F., Naylor H. B. 1959; Electron microscopy of ultrathin sections of schizosaccharo-mycesoctosporus. I. Cell division. Journal of Bacteriology 78:868–877
    [Google Scholar]
  6. Davies R. 1966; Mercaptoethanol and protoplast formation and invertase release from Saccharo-mycesfragilis and other yeasts.. In Symposium on Yeast Protoplasts Abhandlungen der DeutschenAkademie der Wissenschaftenzu Berlin, nr 6 pp 195–198 and 381–382 Müller R. Edited by Berlin: Akademie-Verlag;
    [Google Scholar]
  7. Davies R., Elvin P. A. 1964; The effect of β-mercaptoethanol on release of invertase and formation of protoplasts of Saccharomyces fragilis . Biochemical Journal 93:8P
    [Google Scholar]
  8. Gascon S., Neumann N. P., Lampen J. O. 1968; Comparative study of the properties of the purified internal and external invertases from yeast. Journal of Biological Chemistry 243:1573–1577
    [Google Scholar]
  9. Gerhardt P., Judge J. A. 1964; Porosity of isolated cell walls of Saccharomyces cerevisiae and Bacillus megaterium . Journal of Bacteriology 87:945–951
    [Google Scholar]
  10. Glauert A. M., Thornley M. J. 1966; Glutaraldehyde fixation of Gram-negative bacteria. Journal of the Royal Microscopical Society 85:449–453
    [Google Scholar]
  11. Heppel L. A. 1967; Selective release of enzymes from bacteria. science; New York: 1581451–1455
    [Google Scholar]
  12. Kidby D. K., Davies R. 1968; Thiol-disulphide interaction in yeast cell wall. Journal of General Microbiology 53:v
    [Google Scholar]
  13. Kidby D. K., Davies R. 1970; Thiol-induced release of invertase from cell walls of Saccharomyces fragilis . Biochimica et Biophysica Acta 201:261–266
    [Google Scholar]
  14. Kidby D. K., Goodchild D. J. 1966; Host influence on the ultrastructure of root nodules of Lupinusluteus and Ornithopussativus . Journal of General Microbiology 45:147–152
    [Google Scholar]
  15. Lampen J. O. 1968; External enzymes of yeast: their nature and formation. Antonie van Leeuwenhoek 34:1–18
    [Google Scholar]
  16. Mitchell P. 1959; Biochemical cytology of micro-organisms. Annual Review of Microbiology 13:407–440
    [Google Scholar]
  17. Myrback K. 1960; Invertases. In The Enzymes IV pp 379–396 Boyer P. D., Lardy H., Myrback K. Edited by New York: Academic Press;
    [Google Scholar]
  18. Neu H. C., Chou J. 1967; Release of surface enzymes in Enterobacteriaceae by osmotic shock. Journal of Bacteriology 94:1934–1945
    [Google Scholar]
  19. Nickerson V. J. 1963; Symposium on biochemical bases of morphogenesis in fungi. IV. Molecular bases of form in yeasts. Bacteriological Reviews 27:305–324
    [Google Scholar]
  20. Payne J. W., Gilvarg C. 1968; Size restriction on peptide utilization in Escherichia coli . Journal of Biological Chemistry 243:6291–6299
    [Google Scholar]
  21. Phaff H. J. 1963; Cell wall of yeasts. Annual Review of Microbiology 17:15–30
    [Google Scholar]
  22. Rogers D. 1968; Osmotic pools in Escherichia coli . science; New York: 159531–532
    [Google Scholar]
  23. Sargent M. S., Ghosh B. K., Lampen J. O. 1968; Characteristics of penicillinase release by washed cells of Bacillus licheniformis . Journal of Bacteriology 96:1231–1239
    [Google Scholar]
  24. Snyder H. E., Phaff H. J. 1960; Studies on a β-fructosidase (inulinase) produced by Saccharo-mycesfragilis . Antonie van Leeuwenhoek 26:433–452
    [Google Scholar]
  25. Trevithick J. R., Metzenberg R. L. 1966; Molecular sieving by Neurospora cell walls during secretion of invertaseisozymes. Journal of Bacteriology 92:1010–1015
    [Google Scholar]
  26. Trevithick J. R., Metzenberg R. L., Costello D. F. 1966; Genetic alteration of pore size and other properties of the Neurospora cell wall. Journal of Bacteriology 92:1016–1020
    [Google Scholar]
  27. Weimberg R., Orton W. L. 1966; Elution of exocellular enzymes from Saccharomyces fragilisand Saccharomyces cerevisiae . Journal of Bacteriology 91:1–13
    [Google Scholar]
  28. Weinbaum G., Rich R., Fischman D. A. 1967; Enzyme-induced formation of spheres from cells and envelopes of Escherichia coli . Journal of Bacteriology 93:1693–1698
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-61-3-327
Loading
/content/journal/micro/10.1099/00221287-61-3-327
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error