1887

Abstract

-Glutamic acid (-glu) inhibited strongly the growth of two strains of in a minimal medium. The inhibition was completely annulled by equivalent concentrations of -glutamic acid (-glu) or -gluta-mine. -G1u also inhibited glutamate dehydrogenase (GDH) and was only antagonized completely by 10 equivalents of -glu. -G1u in media containing -glu increased GDH-activity, presumably by de-repression. -G1u showed no effect on glutamine synthetase and γ-glutamyl transferase activities. Inhibition of growth of by -glu thus seems due to the interference with glutamate synthesis by inhibition of GDH-activity.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-61-2-255
1970-05-01
2022-01-19
Loading full text...

Full text loading...

/deliver/fulltext/micro/61/2/mic-61-2-255.html?itemId=/content/journal/micro/10.1099/00221287-61-2-255&mimeType=html&fmt=ahah

References

  1. Beadle G. W. L., Tatum E. L. 1941; Genetic control of biochemical reactions in Neurospora. Proceedings of the National Academy of Sciences of the United States of America 27:499
    [Google Scholar]
  2. Fincham J. R. S. 1951a; Transaminases in Neurosporacrassa . Nature; London: 168957
    [Google Scholar]
  3. Fincham J. R. S. 1951b; The occurrence of glutamic dehydrogenase in Neurospora and its apparent absence in certain mutant strains. Journal of General Microbiology 5:793
    [Google Scholar]
  4. Fox S. W., Fling M., Bollenback G. W. 1944; Inhibition by bacterial growth by d-leucine. Journal of Biological Chemistry 155:465
    [Google Scholar]
  5. Gornall A. G., Bardawill C. J., David M. M. 1949; Determination of serum proteins by means of the biuret reaction. Journal of Biological Chemistry 177:751
    [Google Scholar]
  6. Grossowicz N., Halpern Y. S. 1956; Inhibition of l-asparaginase in extracts of Mycobacterium phleiby d-asparagine. Nature; London: 177625
    [Google Scholar]
  7. Grossowicz N., Halpern Y. S. 1957; Enzymatic transfer and hydrolysis involving glutamine and asparagine. Journal of Biological Chemistry 228:643
    [Google Scholar]
  8. Horowitz N. H., Beadle G. W. 1943; A microbiological method for the determination of choline by use of a mutant of Neurospora. Journal of Biological Chemistry 150:325
    [Google Scholar]
  9. Krebs H. A., Lowenstein J. M. 1960; The tricarboxylic acid cycle. In Metabolic Pathways 1 p. 129 Greenberg D. M. Edited by Greenberg, New York and London: Academic Press;
    [Google Scholar]
  10. Lester G., Yanofsky C. 1961; Influence of 3-methylanthranilic and anthranilic acids on the formation of tryptophan synthetase in Escherichia coli . Journal of Bacteriology 81:81
    [Google Scholar]
  11. Lineweaver H., Burk D. 1934; The determination of enzyme dissociation constants. Journal of the American Chemical Society 56:658
    [Google Scholar]
  12. Olson J. A., Anfinsen C. B. 1953; Kinetics and equilibrium studies on crystalline l-glutamic acid dehydrogenase. Journal of Biological Chemistry 202:841
    [Google Scholar]
  13. Prescott J. M., Schweigert B. S., Lyman C. M., Kuiken K. A. 1949; The effect of d-trypto-phane on the utilization of the l-isomer by some lactic acid bacteria. Journal of Biological Chemistry 178:727
    [Google Scholar]
  14. Roberts E. 1953; Further studies of inhibition of bacterial glutamine decarboxylase. Journal of Biological Chemistry 202:359
    [Google Scholar]
  15. Sanwal B. D., Lata M. 1962a; Effect of glutamic acid on formation of two glutamic acid dehydrogenases of Neurospora. Biochemical and Biophysical Research Communications 6:404
    [Google Scholar]
  16. Sanwal B. D., Lata M. 1962b; Concurrent regulation of glutamic acid dehydrogenase of Neurospora. Archives of Biochemistry and Biophysics 97:582
    [Google Scholar]
  17. Strauss B. S. 1955; Studies on the metabolism of acetate by acetate-requiring mutants of Neurosporacrassa . Archives of Biochemistry and Biophysics 55:77
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-61-2-255
Loading
/content/journal/micro/10.1099/00221287-61-2-255
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error