1887

Abstract

SUMMARY: Methods were developed for growing protoplasts or L bodies of into L colonies on membrane filters. Only some types of filters permitted growth; for optimum growth the filters usually had to be extracted with 2 % (v/v) ethanol in water. Scoring was greatly improved by staining. Growth on the filters induced reversion of the protoplasts to the bacillary form. Reversion was greatly enhanced when wall was added to the filter. A lesser enhancement occurred when wall was added to protoplasts growing in agar. The stimulation of reversion by wall was non-specific since similar stimulation could be obtained by intact autoclaved pseudomonads and yeast. Stimulation of reversion probably depended on physical contact between the naked protoplasts or L bodies and the surface provided by the filter, wall, or killed organism. The present status of induced reversion in is discussed.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-61-2-233
1970-05-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/micro/61/2/mic-61-2-233.html?itemId=/content/journal/micro/10.1099/00221287-61-2-233&mimeType=html&fmt=ahah

References

  1. Altenbern R. A. 1963; Reversion of L forms and spheroplasts of Proteus mirabilis . Journal of Bacteriology 85:269
    [Google Scholar]
  2. Altenbern R. A., Landman O. E. 1960; Growth of L forms of Proteus mirabilis in liquid media. Journal of Bacteriology 79:510
    [Google Scholar]
  3. Bond E. 1969 Gene activity following cell wall removal in the wild type and in a mutant of Bacillus subtilis capable of growth in liquid media in the L-form Ph.D Dissertation Georgetown University:
    [Google Scholar]
  4. Cahn R. D. 1967; Detergents in membrane filters. Science; New York: 155195
    [Google Scholar]
  5. Dienes L. 1968; Morphology and reproductive processes of bacteria with defective cell wall. In Microbial Protoplasts, SpheroplastsandL-Forms p. 74 Guze L. B. Edited by Baltimore: Williams and Wilkins;
    [Google Scholar]
  6. Dienes L., Madoff S. 1966; Development and growth of L forms of bacteria and PPLO on membrane filters. Proceedings of the Society for Experimental Biology and Medicine 121:334
    [Google Scholar]
  7. Dienes L., Weinberger H. J. 1951; The L forms of bacteria. Bacteriological Reviews 15:245
    [Google Scholar]
  8. Landman O. E. 1968; Protoplasts, spheroplasts and L forms viewed as a genetic system. . In Microbial Protoplasts, Spheroplasts and L-Forms p. 319 Guze L. B. Edited by Baltimore: Williams and Wilkins;
    [Google Scholar]
  9. Landman O. E., Altenbern R. A., Ginoza H. S. 1958; Quantitative conversion of cells and protoplasts of Proteus mirabilis and Escherichia coli to the L-form. Journal of Bacteriology 75:567
    [Google Scholar]
  10. Landman O. E., Forman A. 1969; Gelatin-induced reversion of protoplasts of Bacillus subtilisto the bacillary form: Biosynthesis of macromolecules and wall during successive steps. Journal of Bacteriology 99:576
    [Google Scholar]
  11. Landman O. E., Halle S. 1963; Enzymically and physically induced inheritance changes in Bacillus subtilis . Journal of Molecular Biology 7:721
    [Google Scholar]
  12. Landman O. E., Ryter A., Fréhel C. 1968; Gelatin-induced reversion of protoplasts of Bacillus subtilis to the bacillary form: electron-microscopic and physical study. Journal of Bacteriology 96:2154
    [Google Scholar]
  13. Lederberg J., St Clair J. 1958; Protoplasts and L-type growth of Escherichia coli . Journal of Bacteriology 75:143
    [Google Scholar]
  14. McQuillen K. 1956; Capabilities of bacterial protoplasts. Symposia of the Society for General Microbiology 6:127
    [Google Scholar]
  15. Martin H. H. 1963; Bacterial protoplasts-a review. Journal of Theoretical Biology 5:1
    [Google Scholar]
  16. Miller I. L., Wiebe W., Landman O. E. 1968; Gelatin-induced reversion of protoplasts of Bacillus subtilis to the bacillary form: photomicrographic study. Journal of Bacteriology 96:2171
    [Google Scholar]
  17. Miller I. L., Zsigray R. M., Landman O. E. 1967; The formation of protoplasts and quasi-spheroplasts in normal and chloramphenicol-pretreated Bacillus subtilis . Journal of General Microbiology 49:513
    [Google Scholar]
  18. Pelczar M. J. Jun. 1965 In Laboratory Exercises in Microbiology, 2nd ed.. p. 339 New York: McGraw-Hill;
    [Google Scholar]
  19. Young F. E. 1966; Autolytic enzyme associated with cell walls of Bacillus subtilis . Journal of Biological Chemistry 241:3462
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-61-2-233
Loading
/content/journal/micro/10.1099/00221287-61-2-233
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error