Methods were developed for growing protoplasts or L bodies of into L colonies on membrane filters. Only some types of filters permitted growth; for optimum growth the filters usually had to be extracted with 2% (v/v) ethanol in water. Scoring was greatly improved by staining. Growth on the filters induced reversion of the protoplasts to the bacillary form. Reversion was greatly enhanced when wall was added to the filter. A lesser enhancement occurred when wall was added to protoplasts growing in agar. The stimulation of reversion by wall was non-specific since similar stimulation could be obtained by intact autoclaved , pseudomonads and yeast. Stimulation of reversion probably depended on physical contact between the naked protoplasts or L bodies and the surface provided by the filter, wall, or killed organism. The present status of induced reversion in is discussed.


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