1887

Abstract

SUMMARY: Breakdown of DNA to acid-soluble fragments induced in strains of b by nalidixic acid (10 g./ml.) differed in linear rate according to the ability of the strain to repair DNA lesions. Strains which were (-2) and (-i) exhibited excessive DNA breakdown following nalidixic add treatment. The excision-defective strains, -8, -12 and wwp-2 also degraded their DNA to acid-soluble fragments at a rate which initially was greater than that of the parental strain ( ). This degradation was unaffected by the mutation. Removal of nalidixic acid from the system considerably lowered the rate of DNA breakdown. The responses to nalidixic acid and other agents potentiating DNA breakdown are compared.

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/content/journal/micro/10.1099/00221287-61-1-21
1970-04-01
2022-01-17
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References

  1. Aoki S., Boyce R. P., Howard-Flanders P. 1966; Sensitization of Escherichia coli to radiation by bromouracil: Excessive post-irradiation breakdown of DNA without concomitant synthesis. Nature 209:686
    [Google Scholar]
  2. Cook T. M., Brown K. G., Boyle J. V., Goss W. A. 1966; Bactericidal action of nalidixic acid on Bacillus subtilis. Journal of Bacteriology 92:1510
    [Google Scholar]
  3. Donch J., Greenberg J. 1968; Genetic studies of strain B3–8 of Escherichia coli. Genetical Research 2:55
    [Google Scholar]
  4. Goss W. A., Deitz W. H., Cook T. M. 1964; Mechanism of action of nalidixic acid on Escherichia coli. Journal of Bacteriology 88:1112
    [Google Scholar]
  5. Goss W. A., Deitz W. H., Cook T. M. 1965; Mechanism of action of nalidixic acid on Escherichia coli. 11. Inhibition of desoxyribonucleic acid synthesis. Journal of Bacteriology 92:1068
    [Google Scholar]
  6. Grigg G. W. 1968; Caffeine-death and a theory of chromosome breakage: An excision-repair suicide model. In Replication and Recombination of Genetic Material Canberra: Australian Academy of Science;
    [Google Scholar]
  7. Grigg G. W. 1969; Induction of DNA breakdown and death in Escherichia coli by phleomycin. Its association with dark-repair processes. Molecular and General Genetics 104:1
    [Google Scholar]
  8. Howard-Flanders P., Boyce R. P. 1966; DNA repair and genetic recombination.Studies on mutants of Escherichia coli defective in these processes. Radiation Research Suppl 6:156
    [Google Scholar]
  9. Mattern I. E., Zwenk H., Rörsch A. 1966; The genetic constitution of the radiation sensitive mutant Escherichia coli B3–8. Mutation Research 3:374
    [Google Scholar]
  10. Okada F. J., Yanagisawa K., Ryan F. J. 1960; Elective production of thymineless mutants. Nature 188:340
    [Google Scholar]
  11. Rupp W. D., Howard-Flanders P. 1968; Discontinuities in the DNA synthesised in an excision-defective strain of Escherichia coli following ultraviolet radiation. Journal of Molecular Biology 31:291
    [Google Scholar]
  12. Setlow R., Carrier W. L. 1964; The disappearance of thymine dimers from DNA: an error correcting mechanism. Proceedings of the National Academy of Sciences of the United States of America 51:226
    [Google Scholar]
  13. Setlow R. B., Carreer W., Williams R. 1967; Strand breaks during excision of pyrimidine dimers in vivo. Abstracts.Biophysics U.S TG-2 p. 83
    [Google Scholar]
  14. Suzuki K., Moriguchi F., Horii Z. 1966; Stability of DNA in Escherichia colib/rand bs-i irradiated with ultraviolet light. Nature 212:1265
    [Google Scholar]
  15. Witkin E. M. 1968; The role of DNA repair and recombination in mutagenesis. . In Proceedings of the Xllth International Congress of Genetics, Tokyo
    [Google Scholar]
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