1887

Abstract

When is grown in the presence of some antibiotics (Provasoli, Hutner & Schatz, 1948 Ebringer, 1962, 1966 Celmer & Ebringer, 1967 ), organisms with no chloroplasts are soon produced. These aplastidic organisms can then be isolated and maintained as permanently colourless races. The mechanism of the bleaching action is still in dispute. We have suggested an interaction with plastid DNA or some hereditary apparatus responsible for chloroplast replication (Ebringer, Mego & Jurasek, 1969 ). To test this hypothesis, we have examined some antibacterial agents which inhibit DNA synthesis in bacteria or in other organisms. Nalidixic acid inhibits specifically the synthesis of DNA in bacteria (Goss, Deitz & Cook, 1965 Cook 1966 ) and exerts a mutagenic effect upon proliferating bacteria (Cook, Goss & Deitz, 1966 ). Some other agents which affect DNA synthesis in bacteria also exert a bleaching action on (to be published), so the question arises whether nalidixic acid is able to produce permanent colourless races of this organism.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-61-1-141
1970-04-01
2024-12-14
Loading full text...

Full text loading...

/deliver/fulltext/micro/61/1/mic-61-1-141.html?itemId=/content/journal/micro/10.1099/00221287-61-1-141&mimeType=html&fmt=ahah

References

  1. Celmer W. D., Ebringer L. 1967; Effects of certain O-acyl derivatives of oleandomycin and erythromycin on chloroplasts of Euglena gracilis . Journal of Protozoology 14:263
    [Google Scholar]
  2. Cook T. M., Goss W. A., Deitz W. H. 1966; Mechanism of action of nalidixic acid on Escherichia coli. V. Possible mutagenic effect. Journal of Bacteriology 91:780
    [Google Scholar]
  3. Cook T. M., Brown K. G., Boyle J. V., Goss W. A. 1966; Bactericidal action of nalidixic acid on Bacillus subtilis . Journal of Bacteriology 92:1510
    [Google Scholar]
  4. Ebringer L. 1962; Erythromycin and streptomycin-like antibiotics as bleaching factors for Euglena gracilis . Naturwissenschaften 49:334
    [Google Scholar]
  5. Ebringer L. 1966; Antibiotics and apochlorosis. I. Macrolide antibiotics-their common molecular structure responsible for bleaching of Euglena gracilis . Folia Microbiologica, Praha 11:379
    [Google Scholar]
  6. Ebringer L., Mego J. L., Jurášek A. 1969; Mitomycins and the bleaching of Euglena gracilis . Archiv für Mikrobiologie 64:229
    [Google Scholar]
  7. Ebringer L., Mego J. L., Jurášek A., Kada R. 1969; The action of streptomycins on the chloroplast system of Euglena gracilis . Journal of General Microbiology 59:203
    [Google Scholar]
  8. Goss W. A., Deitz W. H., Cook T. M. 1965a; Mechanism of action of nalidixic acid on Escherichia coli . Journal of Bacteriology 88:1112
    [Google Scholar]
  9. Goss W. A., Deitz W. H., Cook T. M. 1965b; Mechanism of action of nalidixic acid on Escherichia coli. II. Inhibition of deoxyribonucleic acid synthesis. Journal of Bacteriology 89:1068
    [Google Scholar]
  10. Mereschkowski C. 1905; Über Natur und Ursprung der ChromatophorenimPflanzenreiche. BiologischesZentralblatt 25:593
    [Google Scholar]
  11. Provasoli L., Hutner S. H., Schatz A. 1948; Streptomycin-induced chlorophyll-less races of Euglena. Proceedings of the Society for Experimental Biology and Medicine 69:279
    [Google Scholar]
  12. Stern J. L., Barner H. D., Cohen S. S. 1966; The lethality of streptomycin and the stimulation of RNA synthesis in the absence of protein synthesis. Journal of Molecular Biology 17:188
    [Google Scholar]
/content/journal/micro/10.1099/00221287-61-1-141
Loading
/content/journal/micro/10.1099/00221287-61-1-141
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error