Autolysis of Extracellular Glucans Produced in vitro by a Strain of Free

Abstract

SUMMARY: usually produces a stable viscous glucan during submerged culture fermentations. A new strain 139/2/1 G of the fungus, which subsequently autolysed this glucan to glucose, was studied and the autolysis ascribed to a constitutive β1 3 glucanase and a β-glucosidase which were detected as soon as the fungal hyphae differentiated to a sclerotial form. The glucanase production followed a sigmoid pattern, reaching a maximum within 12 days, and the liberated glucose contributed to renewed growth towards the end of the fermentation. A sucrase and a maltase were also detected. Maximum glucan autolysis was achieved by using a large spore inoculum. This maintained minimal viscocity throughout the fermentation and, by maintaining adequate aeration, has since facilitated ergot alkaloid production by the organism.

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1970-03-01
2024-03-29
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References

  1. Bock R. M., Ling N.-S. 1954; Devices for gradient elution in chromatography.. Analytical Chemistry 26:1543
    [Google Scholar]
  2. Buck K. W., Chen A. W., Dickerson A. G., Chain E. B. 1968; Formation and structure of extracellular glucans produced by Claviceps species.. Journal of General Microbiology 51:337
    [Google Scholar]
  3. Chesters C. G. C., Bull A. T. 1963; The enzymatic degradation of laminarin.. Biochemical Journal 86:31
    [Google Scholar]
  4. Davis E. N., Rhodes R. A., Shulke H. R. 1965; Fermentative production of exocellular glucans by fleshy fungi.. Applied Microbiology 13:267
    [Google Scholar]
  5. Garza A. C., Weissler H. E. 1967; Automatic simultaneous multiple chemical analyses of worts and beers.. Technicon Symposium 1:201
    [Google Scholar]
  6. Mantle P. G. 1968; Inhibition of lactation in mice following feeding with ergot sclerotia (Claviceps fusiformis (Loveless)) from the bulrush millet (Pennisetum typhoides (Staph and Hubbard)) and an alkaloid component.. Proceedings of the Royal Society B 170:423
    [Google Scholar]
  7. Marks V., Lloyd K. 1963; The enzymatic measurement of glucose by autoanalyser.. Proceedings of the Association of Clinical Biochemistry 2:176
    [Google Scholar]
  8. Reese E. T., Mandels M. 1959; β-d-1,3-glucanases in fungi.. Canadian Journal of Microbiology 5:173
    [Google Scholar]
  9. Roberts R. B., Abelson P. H., Cowie D. B., Bolton E. T., Britten R. J. 1955; Studies of biosynthesis in Escherichia Coli. . Publications, Carnegie Institution of Washington, no. 607
    [Google Scholar]
  10. Stoll A., Brack A., Kobel A., Hofmann A., Brunner R. 1954; Die Alkaloide eines Mutter-kornpilzes von Pennisetum typhoideum, Rich und deren Bildung in saprophytischer Kultur.. Helvetica Chimica Acta 37:1815
    [Google Scholar]
  11. Warburg O., Christian W. 1942; Isolierung und Kristallisation des Garungsferments Enolase.. Biochemische Zeitschrift 310:384
    [Google Scholar]
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